摘要
目的:观察丹参酮IIA磺酸钠(STS)对血管紧张素II(Ang II)诱导的心肌肥大及p-ERK表达的影响。方法:培养新生大鼠心肌细胞,考马斯亮蓝法测定心肌细胞蛋白含量、[3H]-亮氨酸掺入法测定蛋白合成速率作为心肌肥大指标;用West-ern-blot测定p-ERK表达。结果:STS能显著降低Ang II诱导的心肌细胞总蛋白含量、蛋白合成速率上升,同时对p-ERK表达具有剂量、时间依赖性抑制作用。结论:STS可以抑制Ang II诱导的心肌肥大,机制与抑制p-ERK表达有关。
OBJECTIVE To observe effects of sodium tanshinone IIA sulfonate （STS） on angiotension II（ Ang II）-induced cardiomyocyte hypertrophy and the expression of phosphorylated extracellular signal-regulated kinase （p-ERK）. IVIETHODS In the primary culture of neonatal rat cardiomyocytes, as indexes of cardiomyocyte hypertrophy, the total protein was determined by Coomassie brilliant blue and protein synthesis rate was measured by [^3 H]-Leucine incorporation. The expression of p-ERK was assessed using Western blot. RESULTS STS can decrease Ang II-induced elevations of the total protein and protein synthesis rate; and inhibit the expression of p-ERK in a dose- and time-dependent manner. ClDNCLUSION The anti-hypertrophic effects of STS on cardiomyocyte hypertrophy induced by Ang II are associated with inhibition of ERK signaling pathway.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2006年第10期1191-1194,共4页
Chinese Journal of Hospital Pharmacy
基金
国家自然科学基金资助项目(编号:30500657)
关键词
丹参酮ⅡA磺酸钠
心肌肥大
细胞外信号调节激酶
sodium tanshinone IIA sulfonate
cardiomyocyte hypertrophy
extracellular signal-regulated kinase
