摘要
以带节茎段为外植体进行了白萼吊钟海棠（Fuchsia alba—coccinea Hort．）的组织培养和快速繁殖研究，对外植体的灭菌方法以及在试管苗增殖和生根培养过程中不同浓度的激素配比进行了筛选，同时研究了抑制试管苗褐化和玻璃化的方法。结果表明，最适宜白萼吊钟海棠外植体灭菌的方法是用0．1％HgCl2处理4～6min；MS培养基中不加NH4NO3可完全消除试管苗玻璃化现象；MS培养基中加入1．0g·L^-1PVP，可基本抑制试管苗褐化；试管苗增殖的最佳培养基为含0．8mg·L^-16-BA、0．10mg·L^-1NAA和1．0g·L^-1PVP的MS培养基；试管苗生根的最适培养基为含0．1～0．2mg·L-1NAA和1．0g·L^-1PVP的1／2Ms培养基。
By using explants of stem fragments with nodes, the tissue culture and rapid propagation of Fuchsia alba-coccinea Hort. were carried out. The results showed that the optimal time for surfacesterilization of explants with 0.1% HgCl2 was 4 - 6 min. The best multiplication effect could be achieved when induced in medium MS containing 0.8 mg ·L^-1 6-BA, 0.10 mg ·L^-1 NAA and 1.0 g·L^-1 PVP. The best effect was appeared when shoots rooted on medium 1/2 MS with 0.1 -0.2 mg·L^-1 NAA and 1.0 g ·L^-1 PVP. The vitrifying of plantlets could be completely controlled when NH4 NO3 was removed from medium MS. The browning of plantlets could be generally controlled when 1.0 g ·L^-1 PVP was supplemented into medium MS.
出处
《植物资源与环境学报》
CAS
CSCD
2006年第3期55-59,共5页
Journal of Plant Resources and Environment
基金
苏州市农业科技发展计划攻关项目(SNZ-0305)
关键词
白萼吊钟海棠
组织培养
玻璃化
褐化
Fuchsia alba-coccinea Hort.
tissue culture
vitrifying
browning
