摘要
为研究江浙地区杨梅(Myrica rubraS ieb.et Zucc.)的亲缘关系,运用ISSR-PCR分析方法对其主要栽培品种进行了分析。8条引物扩增出58个DNA片段,其中多态性片段42个,占总扩增片段的72.4%。结合遗传距离分析,运用UPGMA法构建了分子系统树。结果表明,所分析的14个品种和1个优良单株以0.14为结合线可分为5组,说明江浙地区的杨梅在广泛基因交流的情况下,存在局部的隔离。‘小叶细蒂’与‘极早熟1号’亲缘关系较近,前者可能是后者的亲本之一。
Inter-simple sequence repeat（ISSR） was used to analysis genomic DNA variations and genetic relationships of waxberry （ Myrica rubra Sieb. et Zucc. ） in Jiangsu and Zhejiang provinces. 8 of 11 ISSR primers were proved to be polymorphic and informative. Total 58 DNA fragments were amplified and 42 were polymorphic（72.5% ）. The genetic distance was analysed according ISSR result and a dendrogram was constructed using UPGMA method. All cuhivars tested were classified into 5 groups and the excellent individual （Jizaoshu No. 1 ） has a close relationship with ＇ Xiaoyexidi＇. This indicates that there were a wide gene flow and topical isolation between waxberry cultivars of two provinces. Maybe ＇ Xiaoyexidi＇ was a parent of ＇Jizaoshu No. 1 ＇.
出处
《植物资源与环境学报》
CAS
CSCD
2006年第3期17-20,共4页
Journal of Plant Resources and Environment
关键词
杨梅
ISSR
品种鉴定
亲缘关系
waxberry （Myrica rubra Sieb. et Zucc. ）
ISSR
cuhivar identification
genetic relationship
