摘要
目的探讨聚羟基丁酸酯-羟基戊酸酯（PHBV）作为软骨细胞支架的可行性。方法采用“压片-热处理-粒子析出”技术制备PHBV多孔支架并观测其结构。体外分离培养软骨细胞后接种到PHBV支架进行培养，第3、7、14天后取材行扫描电镜观察；培养至2、6、10周后行组织学观察。结果PHBV支架孔径在200-300μm之间孔隙率80％；软骨细胞-PHBV复合物在体外培养期间支架无塌陷及形变，软骨细胞在支架上黏附、增殖良好，并能分泌细胞外基质。随着培养时间延长，新生软骨的组织学特征日益明显。结论PHBV支架具有适宜的孔结构和生物相容性，可以作为软骨组织工程支架材料。
Objective To investigate the possibility of poly hydroxybutyrate-co-hydroxyvalerate （PHBV） as scaffolds for chondrocytes. Methods PHBV porous scaffolds were fabricated using a compression moulding, thermal processing and salt particulate leaching method. The porous structure of the scaffolds was investigated with optical microscope （OM） and scanning electron microscope （SEM） and the porosity was calculated. Chondrocytes isolated from articular cartilage were seeded into PHBV scaffolds and cultured in vitro. After incubation for 3,7 and 14 days, the chondrocytes on the scaffolds were examined by SEM, and the cell-scaffolds cultured in vitro for 2,6 and 10weeks were examined histologically. Results The pore size in the scaffolds ranged 200-300μm and the porosity was about 80%. During the culture periods, the specimens maintained their original shapes. SEM showed that the chondrocytes could aggregate and synthesize extracellular matrix on PHBV scaffolds. Histological examination confirmed that morphology of the chondrocytes and the expression of the cartilaginous matrices and characteristics of the engineered cartilage generated in vitro were more typical with increased culture period. Conclusion PHBV scaffold is suitable in cartilage tissue engineering as it has optimal porous structure and biocompatibility.
出处
《江苏医药》
CAS
CSCD
北大核心
2006年第10期943-945,F0002,共4页
Jiangsu Medical Journal
