摘要
目的:观察X射线照射对小鼠腹腔巨噬细胞IL-18表达的影响。方法:昆明种小白鼠80只,随机分为10组(每组8只),即假照组、4个单次低剂量X射线照射组(0·05、0·075、0·1和0·2Gy)和5个单次高剂量X射线照射组(0·5、1·0、2·0、4·0及6·0Gy),X线全身照射(WBI)后16h处死小鼠,取腹腔巨噬细胞,用RPMI1640培养基,在37℃、5%CO2培养箱中培养72h,留取上清液,采用ELISA法检测小鼠腹腔巨噬细胞IL-18的分泌量。结果:0·05GyX射线WBI后,IL-18的表达有所增高,检测值为(59·53±3·68)ng·L-1,与假照组(51·29±3·26)ng·L-1比较差异无显著性(P>0·05);0·075GyX射线WBI后,IL-18的表达量增高至(122·78±8·07)ng·L-1,与假照组比较差异有显著性(P<0·01);随着X射线辐射剂量增加至0·1、0·2、0·5和1·0Gy,IL-18的表达量增高至(135·32±5·27)、(149·40±16·54)、(200·42±15·42)及(347·59±17·88)ng·L-1,1·0Gy照射达到峰值,随后IL-18表达呈下降趋势;2·0、4·0及6·0GyWBI后,IL-18的表达量分别为(229·49±14·68)、(253·79±6·69)及(223·32±20·85)ng·L-1,均高于假照组(P<0·01)。结论:高、低剂量X射线照射均能诱导小鼠腹腔巨噬细胞IL-18的表达。
Objective To observe the effect of X-rays irradiation on IL-18 expression in mouse peritoneal macrophages. Methods Eiyghty Kunming white mice were divided into 10 groups （ eight each group） ： shamirradiation, four groups of low-dose irradiation （0.05, 0.075, 0.1 and 0.2 Gy）, and five groups of high-dose irradiation （0.5, 1.0, 2.0, 4.0 and 6.0 Gy）. The mice were killed 16 h after whole body irradiation （WBI）, with X-rays then the peritoneal macrophages were obtained and cultivated with culture medium of RPMI 1640 in culture box with 37℃, 5% CO2 for 72 h. The supernate was gathered to detect the IL-18 expression level in macrophages by ELISA. Results The IL-18 expresson level in sham-irradiation group was （51.29 ± 3.26 ） ng·L^-1, IL-18 expression was enhanced after 0. 05 Gy WBI, but there was no significant difference （P〉0. 05）. The expression level was increased to （122.78±8.07） ng ·L^-1 after 0. 075 Gy WBI, and there was significant difference compared with sham-irridiation group （P〈0.01）. IL-18 expression was increased gradually with the enhancement of X-rays irradiated doses （0.1, 0.2, 0.5 and 1.0 Gy）, and the values were （135.32±5.27）, （149.40± 16.54）.（200. 42±15. 42） and （347.59 ± 17.88） ng·L^-1, respectively. It peaked after 1.0 Gy irradiation, then it decreased after 2.0, 4.0 and 6.0 Gy irradiation, and the values were （229.49±14.68）, （253. 79 ± 6. 69） and （223.32±20.85） ng·L^-1, respectively, but they were higher than that in sham irradiation group （P〈0.01）. Conclusion Both high-dose and low dose X-rays irradiation can stimulate IL-18 expression in mouse peritoneal macrophages.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2006年第5期759-761,共3页
Journal of Jilin University：Medicine Edition
基金
国家自然科学基金资助课题(30100033)
