摘要
目的:观察中药虎芪活血煎对大鼠肺纤维化的干预作用,并探讨其可能的作用机制。方法:通过气管内注射博莱霉素A5(BLMA5)5mg·kg-1复制大鼠肺纤维化模型,给予小、中、大剂量(分别为10g·kg-1·d-1、20g·kg-1·d-1和40g·kg-1·d-1)的虎芪活血煎干预后,用HE及VG染色观察肺组织病理变化和胶原沉积情况,并计算肺泡炎和肺纤维化积分;用免疫组织化学(IHC)染色测定肺组织转化生长因子-β1(TGF-β1)表达水平,并行图像定量分析。结果:模型对照组肺泡炎和肺纤维化积分及肺组织TGF-β1表达显著高于正常对照组(P<0.01);虎芪活血煎中、大剂量组肺泡炎和纤维化积分及肺组织TGF-β1表达均显著低于模型对照组(P<0.01);而虎芪活血煎小剂量组作用不佳。结论:中、大剂量的虎芪活血煎能减轻肺泡炎和肺纤维化程度,对博莱霉素A5诱导的大鼠肺纤维化有良好的保护作用。其机制可能是通过抑制TGF-β1的表达,减少了胶原沉积,使炎症及纤维化病变减轻。
Objective： To evaluate the effect and mechanism of Huqihuoxue Decoction on bleomycin-induced pulmonary fibrosis in rats. Methods： Firstly, to reproduce rat models of pulmonary fibrosis by injecting bleomycin As （5 mg/kg） into trachea, then to treat the rat models by use of Huqihuoxue Decoction with low - , median - and high-dose （10g·kg^-1·d^-1、20g·kg^-1·d^-140g·kg^-1·d^-1 respectively）, finally, the pulmonary histopathologieal changes and collagen disposition were observed by hematoxylin and eosin （HE） staining and Van Gieson （VG） staining, and the expression of transforming growth factor- 1 （TGF - β1 ） in lungs were measured by immunohistochemieal （IHC） staining. Results： The scores of the alveolitis and pulmonary fibrosis, and the expression of TGF- β1 in lungs were lower in Huqihuoxue Decoction with median - and high-dose group than those in model group （ P 〈 0.01）, while those in Huqihuoxue Decoction with low-dose group comparing with model group were shown no significant difference. Conclusion： Huqihuoxue Decoction with median- and high-dose would ameliorate the alveolitis and pulmonary fibrosis from bleomytin-induced pulmonary fibrosis in rats. Inhibiting the expression of TGF - β1, decreasing collagen deposition in pulmonary tissues may be one of the mechanisms.
出处
《河南中医学院学报》
2006年第6期20-23,共4页
Journal of Henan University of Chinese Medicine
关键词
虎芪活血煎
博莱霉素
肺纤维化
转化生长因子Β1
Huqihuoxue Decoction
Bleomycin
Pulmonary fibrosis
Transforming growth factor-β1
