摘要
研究了不同浓度抗坏血酸(ascorbicacid,AsA)对5mg.L-1Cd2+胁迫下黑藻(Hydrillaverticillata)体内超氧阴离子(O2.-)产生速率、过氧化氢(H2O2)含量、抗坏血酸和谷胱甘肽(GSH)含量,以及超氧化物歧化酶(SOD)、超氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性的影响.结果表明,与单一Cd2+胁迫相比,随着外源AsA浓度的升高,黑藻体内活性氧生成速率逐步降低;抗氧化物质AsA先升后降,GSH缓慢升高;抗氧化酶APX和CAT活性先升后降,POD活性逐步下降,并接近正常状态,对SOD活性影响不大.可见AsA能够有效缓解Cd2+对黑藻的毒害,且60mg.L-1浓度下的缓解效果最好.
In this paper, HydriUa verticiUata was cultured in the hoagland solution containing 5 mg · L^-1 Cd^2＋ and different concentration ascorbic acid （AsA） to investigate the protective action of AsA to aquatic plant suffered from heavy metal＇s stress. The O2^- generation rate, H2O2 content, antioxidants （AsA and GSH） contents, and antioxidant enzymes （SOD, POD, CAT and APX） activities were analyzed. The results showed that compared with single Cd^2＋ treatment, AsA addition lessened the reactive oxygen species （O2^- and H2O2 ） generation rates, endogenous AsA content, and antioxidant enzyme activities. It was concluded that exogenous AsA could relieve the Cd^2 ＋ poison to H. verticiUata, and its optimum concentration was 60 mg· L^-1.
出处
《应用生态学报》
CAS
CSCD
北大核心
2006年第9期1768-1770,共3页
Chinese Journal of Applied Ecology
基金
国家自然科学基金项目(30370083)
江苏省教育厅自然科学基金项目(03KJBI800620)
江苏省重点实验室开放基金资助项目(164070300805).
关键词
抗坏血酸
CD^2+
黑藻
抗氧化酶
Ascorbic acid, Cd^2＋ , Hydrilla verticillata, Antioxidant enzyme.
