摘要
目的:利用双向电泳技术(2-DE)观察吡咯里西啶生物碱isoline致小鼠急性肝损伤后对肝内亲水性蛋白表达的影响。方法:小鼠一次性灌胃给药isoline,通过血清生化指标检测和病理切片观察isoline的急性肝毒性,抽提肝组织亲水性蛋白做双向电泳分析,利用PDQuest软件以正常组作为参考胶进行匹配分析。结果:丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)检测和肝组织病理切片结果表明,口服isoline造成小鼠急性肝损伤,双向电泳图像分析发现与对照组相比,isoline组小鼠肝组织亲水性蛋白表达图谱中有39个蛋白斑点变化差异显著,其中新出现5个蛋白斑点,缺失10个蛋白斑点。结论:Isoline灌胃可以造成小鼠急性肝损伤,其中蛋白双向电泳分析结果中差异表达的蛋白质点可能参与了isoline的急性肝毒性作用。
AIM： To investigate expression changes of hydrophilic proteins in acute injured mouse liver induced by a pyrrolizidine alkaloid Isoline, using two-dimensional electrophoresis technique. METHOD： Isoline was ig. administered to mice at a single dose of 120 mg· kg^-1 to induce hepatotoxicity, assayed by ALT and AST activities in mouse serum and observed by pathological changes of hepatic tissue under microscope. Samples of hydrophilic proteins for 2-DE were prepared using a ReadyPrep Sequential Extraction Kit（Bio-Rad） and subjected to 2-D gel electrophoresis. Gel images were analyzed and matched by PDQuest software （Bio-Rad） in comparsion to vehicle. RESULT： ALT and AST assay and hepatic pathological section observation confirmed that isoline induced acute injury in mice liver. In 2-D electrophoresis maps of isoline-treated group, 39 significantly changed protein spats were observed, which increased and decreased in quantity, including 10 missed and 5 new emerged spots, compared to the maps of vehicle group. CONCLUSION： Administration of isoline can induce acute hepatic injury, in mice. The differently expressed proteins between vehicle group and isolinetreated group may play an important role in this pathologic process.
出处
《中国天然药物》
SCIE
CAS
CSCD
2006年第5期368-372,共5页
基金
国家自然科学基金重点项目资助(NO.30530840)~~
