摘要
本文建立了植物油中α—亚麻酸等不饱和脂肪酸的ＨＰＬＣ测定方法。样品甲酯化后直接测定，恒浓度洗脱。方法的线性范围：５—３０μｇ／２０μｌ，回收率８３．６±０．３％（ｎ＝４），日内、日间变异系数１．２—２．８％。对内蒙产亚麻籽油进行了测定，其中，α—亚麻酸、亚油酸、油酸含量分别为５５％、２２％和１６％左右。该方法简单、实用。
HPLC method was established for the determination of α linolenic acids and otherunsaturated fatty acids in vegetable oils.Samples are directly determined after methy lesterationand eluted by constant mobil phase.The linear range was 5- 30μg/20μl and recovery was83.6±0.8%,the variation coefficients within one day and between days were 1.2～2.3%。The resultsfor the determination of linseed oil from inner Mongolia were about 55 %,22%and 16% forα linolenic acid.linoleic acid and oleic acid respectively。This method is sunple and feasible.
出处
《重庆医科大学学报》
CAS
CSCD
1996年第3期223-225,共3页
Journal of Chongqing Medical University
