摘要
目的探讨慢性活动性乙型肝炎(CAHB)患者外周血活化抗原CD69、HLA-DR分子在T细胞表达的变化及其意义。方法以31例正常人作为对照,采用免疫荧光三色标记流式细胞术,检测30例CAHB患者外周血T细胞亚群及其CD69、HLA-DR抗原表达;同时用荧光定量PCR检测患者的病毒DNA载量。结果CAHB患者外周血CD3+T细胞的百分率(63.78%±9.70%)与正常对照组比较差异有显著性(P<0.01),CD4+T细胞的百分率(45.88%±8.51%)明显低于正常对照组(P<0.001),CD8+T细胞的百分率(44.64%±7.10%)明显高于正常对照组(P<0.001);CD3+T细胞CD69的表达(6.85%±2.83%)与正常人对照组比较差异也有显著性(P<0.01),其中CD4+T细胞CD69的表达(2.31%±0.94%)与正常对照组比较差异无显著(P>0.05),CD8+T细胞表达CD69水平(3.87%±1.81%)明显高于对照组(P<0.01);CD3+T细胞上HLA-DR的表达(17.09%±4.23%)与正常对照组之间差异有显著性(P<0.001),其中CD4+T细胞中HLA-DR+细胞(7.97%±2.43%)和CD8+T细胞中HLA-DR+细胞(5.52%±1.48%)均比正常对照组显著增高(P<0.01)(P<0.001);CD8+CD69+、CD8+HLA-DR+细胞的数量与病毒DNA载量之间呈正相关。结论CAHB患者存在细胞免疫功能紊乱和淋巴细胞异常激活现象,T淋巴细胞的激活以CD8+T细胞为主,其在抗病毒感染中起着重要作用。
Objective To investigate the expression of special marker for activation of peripheral blood T lymphocytes in patients with chronic hepatitis B virus and its significance. Methods Immunofluoreseent three - color flow cytometry was used to study the expression of CD69 and HLA - DR on T lymphocytes in 30 patients with chronic active hepatitis B virus and 31 normal controls. The viral load was determined by fluorescence quantitative PCR. Results Expression of CD3^＋ T ceils were significantly lower in patients（63.78 % ±9.70% ）than that in controls （P 〈 0.01 ）, meanwhile the expression of C D4 ＋ T ceils was 45.88 % ＋ 8.51%, significantly lower than that of the controls （ P 〈 0. 001 ） and the expression of CD8^＋ T ceil was 44.64% ＋ 7.10%, significantly higher than that of the controls （ P 〈 0.001）. Detection of CD69 on CD3^＋ T ceils in patients was 6.85% ± 2.83%, significantly higherthan that of the controls （ P 〈 0.01 ）. NO significant difference in the expression of CD69 on CD4^＋ T ceils was observed between patients and the controls （ P 〉 0.05）, and the expression of CD69 on CD8^＋ T ceils was 3.87% ＋ 1.81% , significantly different from that of the controls （ P 〈 0.01 ）. The expression of HLA - DR on CD3 ＋ T ceils were significance difference between patients （ 17.09 % ± 4.23 % ） and that in controls （ P 〈 0. 001 ）. The percentages of CD4^＋ T ceils expressing HLA - DR in patients was 7.97 % ± 2.43 %, significantly higher than that of the controls （ P 〈 0.01 ）. Significant difference was observed in the expression of HLA - DR on CD8^＋ T ceils in patients（5.52% ± 1.48% ）as compared with controls （ P 〈 0.001）. There were positive correlations between percentages of CD8^＋ CD69^＋ ,CD8^＋ HLA - DR ＋ ceils and viral load. Conclusion The findings support that cellular immunodeficiency exists in patients and that T lymphocytes were abnormally activated in the patients. The activation of peripheral blood T lymphocytes in patients mainly involves CD8 ＋ subset and it may play an important role in the immune response to antiviral infection.
出处
《中国热带医学》
CAS
2006年第9期1543-1545,共3页
China Tropical Medicine
