摘要
研究用红细胞（ＲＢＣ）作为超氧化物歧化酶（ＳＯＤ）载体的可行性，将包载ＳＯＤ的ＲＢＣ（ＲＢＣ－ＳＯＤ）用于家兔脑缺血－再灌注（Ｉ－Ｒ）模型研究。采用５１Ｃｒ标记测量ＲＢＣ的循环半寿期（ ），兔自身ＲＢＣ的，ＲＢＣ－ＳＯＤ的ＲＢＣ－ＳＯＤ对脑Ｉ－Ｒ期间脂质过氧化物（ＬＰＯ）的升高有一定程度的抑制作用。对照组再灌１０ｍｉｎ时，血浆一氧化氮（ＮＯ）浓度达最高；对照组和游离ＳＯＤ组从缺血１０ｍｉｎ到再灌２７０ｍｉｎ，ＮＯ浓度逐渐回落；但ＲＢＣ－ＳＯＤ组在２７０ｍｉｎ的再灌期间，ＮＯ始终处于持续较高浓度状态。提示完整的ＲＢＣ－ＳＯＤ载体能够清除脑Ｉ－Ｒ时产生的超氧阴离子自由基（ ）。
The possibility of using red blood cells(RBC)as the carrier of superoxide dismutase(SOD)was studied in this paper.SOD was encapsulated into rabbit erythrocytes(RBC-SOD),and was iised in the study of cerebral ischemiareperfusion(I-R).The effect of RBC-SOD on cerebial I-R was observed.The circulation half-life()of RBC-SOD was deter-mined in rabbits by 51Cr radiolabel.was 13.4±1.5 days(n=6)for RBC,and was13.7±1.0 days(n=5) for RBC-SOD,The increase of lipid peroxidation(LPO)during cere-bral I-R was inhibited by RBC-SOD,The maximum concentration of nitric oxide (NO)inplasma occurred in 10 minutes following reperfusion;The concentration of nitric oxide re-duced gradually as reperfusion continued in both control and SOD groups,but it remained atarelatively high level continous during the 4.5 hours of reperfusion in RBC-SOD group. These results indicate that intact RBC-SOD could scavenge superoxide anion()producedduring cerebral I-R.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1996年第5期392-396,共5页
Acta Academiae Medicinae Sinicae
基金
中国医学科学院重点项目
关键词
超氧化物歧化酶
红细胞
载体
脑缺血
再灌注
superoxide dismutase
erythrocytes carrier
cerebral ischemia-reperfusion
