摘要
从土壤、水体和受单甲脒长期污染的样品中通过富集培养，从中分离筛选到一株单甲脒耐性较高的ＤＲ－８菌株。该菌株在牛肉汁培养基中对单甲脒的耐性可达１２５０ｍｇ／Ｌ，而在无机盐培养基中的耐性则为５００ｍｇ／Ｌ。该菌株可以利用单甲脒作为唯一氮源，但是不能以单甲脒作为碳源和能源而生长。降解单甲脒的最适温度为３７℃，最适ｐＨ为７．０，其完整细胞悬液对５５０ｍｇ／Ｌ左右单甲脒的降解率最高可达６４．８２％。经鉴定该菌株为门多萨假单胞菌（ＰｓｅｕｄｏｍｏｎａｓｍｅｎｄｏｃｉｎａＤＲ－８）。１ｍｌ。１．１．３无氮培养基：同培养基２，加３．０ｇ葡萄糖，以ＤＭＡ代替ＮＨ＿４Ｃｌ。１．１．４普通牛肉汁培养基：作为收集细胞用。以上培养基在灭菌后加入一定量的单甲脒即为含单甲脒培养基。１．２单甲脒实验中所用的单甲脒为一种市售农药。
A strain of bacter DR-8, capable of tolerance to DMA, was isolated andselected from samples contaminated by DMA. The tolerance concentration of this strain toDMA were 1250mg/l during growth in beef extract medium and only 500mg/l in inorganicmedium. Furthermore, this strain can utilize DMA as sole nitrogen source, but can notutilize DMA as carbon and energy source. The optimum temperature and PH for degradationof DMA by strain DR-8 were 37℃ and 7.0, respectively. The biodegradation rate of DMAat the concentration about 350mg/l by intact cells was 64.82%. Strain DR-8 was identifiedas Pseudomonas mendocina by the Biolog Microstation Identification System.
出处
《微生物学通报》
CAS
CSCD
北大核心
1995年第5期285-288,共4页
Microbiology China
基金
国家自然科学基金
关键词
门多萨假单胞菌
单甲脒
生物降解
环境微生物
Pseudomonas mendocina DR-8, Biodegradation, N-2,4-dimethyl-phenyl-N'-methylformamidine (DMA.)
