摘要
基于在转录因子结合位点各碱基出现的概率不相同,以转录因子结合位点每一位置的碱基保守程度为参量,分别计算每种转录因子结合位点在每一位置的碱基保守指数Mi.通过构建每种转录因子结合位点位置权重矩阵(PWM),利用位置权重矩阵打分函数对酵母四种转录因子结合位点进行预测.利用se lf-cons istency和cross-va lidation两种方法对此算法进行检验,均获得了较高的预测成功率.结果显示四种转录因子结合位点的预测成功率均超过80%,且同时获得多个试验未测定的转录因子结合位点,对实验有指导意义.
Based on the nucleotide frequency in each position of transcription factor binding sites （TFBS） ,the site conservation indexes Mi and a novel position weight matrix （PWM） are calculated and presented for predicting yeast TFBSs. Four types of TFBSs which were known by experiment are used to train and test this algorithm. The self-consistency test and the cross-validation test are used to estimate the predictive capability of PWM. The predictive results show that the overall accuracies for four types of TFBSs are all more than 80%. Some possible TFBSs are predicted in our results.
出处
《内蒙古大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第5期524-530,共7页
Journal of Inner Mongolia University：Natural Science Edition
基金
国家自然科学基金项目(No.30560039)
关键词
转录因子结合位点
位置权重矩阵
碱基保守性指数
transcription factor binding sites
position weight matrices （PWM）
sites conservation indexes
