摘要
实验选用雄性Ｓｐｒａｇｕｅ－Ｄａｗｌｅｙ大鼠研究顺铂肾毒性发生的机理。实验动物静脉注射１０ｍｇ／ｋｇ顺铂后３小时，肾脏光镜与透射电镜检查及肾小球滤过率无明显变化。但顺铂注射后３０分钟，尿钠、钾浓度分别增加５６％及２．６倍，３小时后肾小管细胞线粒体功能明显异常：状态４呼吸上升１倍，状态３呼吸、呼吸控制率及ＦＣＣＰ解偶联呼吸分别抑制４６％、７４％及４７％。揭示线粒体功能障碍可能为大白鼠顺铂肾毒性发生早期的中心环节。
Male Sprague-Dawley rats received an intravenous cisplatin of 10 mg/kg. Three hours following cisplatin administration,no evident morphological abnormalities were found by both light and electron microscopy; there was also no significant changes in glomerular filtration rate, However, 30 minutes after cisplatin injection, urine sodium and potassium concentration increased by 56% and 260% of those in control animals,respectively.Apparent renal mitochondrial respiration dysfunction was encountered in cisplatin-treatd rats 3 hours later: state 4 respiration was doubled and state 3 respiration, respiratory control ration and carbonyl cyanide p-trifluoromethoxyphenylhydrazone-uncoupled respiration decreased by 46%、74% and 47% of those in controls, respectively. The present data suggest that mitochondrial dysfunction may be a very early event in cisplatin nephrotoxicity in rats.
出处
《实用肿瘤杂志》
CAS
北大核心
1996年第6期268-270,共3页
Journal of Practical Oncology
