摘要
目的观察大葱精提物作用于人胃癌细胞MGC803后的形态学改变。方法将大葱分离提取为脂溶性(D组)和水溶性(C组)两部分,以大葱粗提物(B组)作对照组,作用于MGC803后,倒置显微镜、光镜、电镜观察胃癌细胞形态结构变化。结果①倒置显微镜观察,10%大葱粗提物作用16h,癌细胞缩小变圆,24h细胞脱落;0.05%大葱油作用15h,细胞变小变圆,24h脱落。10%大葱水提物作用72h癌细胞变圆脱落。②光镜下HE染色发现大葱油和大葱粗提物作用24h,细胞体积缩小,核质比例减小,分裂相减少;胞质嗜酸性增强,核浓染固缩成球形;而水溶性提取物组作用24h,癌细胞变化不明显。③扫描电镜可见大葱油和大葱粗提物作用后癌细胞表面微绒毛脱落、缩短、变平,树枝状伪足消失;水溶性提取物组作用后癌细胞表面无明显变化。④透射电镜下观察,大葱油和大葱粗提物作用后,均出现典型的凋亡细胞形态学改变;水溶性提取物组作用后癌细胞未出现凋亡变化。结论大葱提取物能明显抑制体外培养的胃癌细胞生长增殖,诱导胃癌细胞凋亡,其起主要作用的部分为脂溶性部分,水溶性部分作用微弱,大葱油中抗癌的确切成分及作用机制有待深入研究。
Objective To observe morphological changes of gastric cancer after treatment with the refined extracts of allium fistulosum. L （ AF ）. Methods Allium was divided into lipid-solution （ group D ） and water-solution （ group C） , and the rawextracts of allium was taken as control（ group B）. The morphological changes of humangastric cancer were observed by inverted microscope, optical microscope and electron microscope after MGC803 was treated with AF. Results 1. By inverted microscope, the cancer cell shrinked and became round when it was treated with 10% raw extracts o [ hllium for 16h and 24h treatment had cell exfoliated ;- J2h use of 0.5 - 0a - m oil had x：ell shrinked and became ro -d and4- e would make it exfoliated; 15 h use of 0.05% allium oil made the cell become small and round and24h use would make it exfoliated. 72 huse of 10% water-solution extract of allium could make cancer cell become round and exfoliated. 2. By HE with optical microscope, cell miotosis decreased, cell volume diminished and nucleus became lunula or ball shape after 24h treatment with allium oil and raw extracts of AF, but the cancer cell was not changed significantly by 24 h treatment of water-solution extract of AF. 3. By scanning electron microscope, microvillus on surface of the ceils were shorten or smoothen ,branch-like pseudopodium disap- peared after treatment with allium oil and raw extracts of AF, but the cancer ccll was not changed by water-solution extract of AF. 4. With transmissJonal electron microscope, cell ultrastructure exposed to allium oil and raw extracts of AF changed as typical apoptosis, but the apoptosis did not appear when it was treated by water-solution extracts. Conclusion The extracts of AF can inhibit the proliferation of gastric cancer cell cultured in vitro and induce apoptosis of gastric cancer cell and lipid portion of extracts is the main effective anti-cancer component but the effect of water portion of extracts is weak. Exact component of allium oil for anti-cancer needs to be further studied.
出处
《中国综合临床》
北大核心
2006年第11期961-963,共3页
Clinical Medicine of China
基金
河北省卫生厅医学科学研究重点资助项目(04041)
关键词
胃癌
大葱精提物
MGC803
凋亡
Gastric cancer
MGC803
Apoptosis
Refined extracts of aUium fistulosum. L
