摘要
目的探讨硫辛酸(LA)对内毒素(脂多糖,LPS)诱导的急性肺损伤(ALI)大鼠白细胞介素(IL)-10含量的影响。方法72只大鼠随机分为4组,对照组、LPS组LPS+LA30mg干预组。LPS+LA100mg干预组。各实验组舌下静脉注射LPS诱导大鼠ALI模型,30min后2个干预组分别给予LA30mg/kg和100mg/kg舌下静脉注射,对照组给予等体积生理盐水。LPS注射1、3、6h后测定血清及肺组织中IL-10浓度。结果各实验组血清及肺组织中IL-10分别在不同时间点显著高于对照组(P<0.01)。2个干预组血清及肺组织中IL-10水平在3h、6h明显升高,均显著高于LPS组(P<0.01),且LPS+LA100mg组显著高于LPS+LA30mg组(P<0.05)。结论LPS可导致ALI的发生,LA可提高IL-10水平,改善肺损伤。
Objective To investigate the effects of hpoicacid （LA）on expression of interleukin-10 （IL-10） in rat with acute lung injury （ALl） induced by lipopolysaccharide（LPS） .Methods 72 rats were randomly divided into four groups （normal group, LPS group, LPS ＋ LA 30 mg group, LPS ＋ LA 100 mg group）. ALl model was made by hypoglossial injection of LPS, and 30 minutes later LA was injected in LPS ＋ LA 30 mg group, and LPS ＋ LA 100 mg goup. At 1,3 and 6h after LPS injection, the lung homogenates and serum were prepared to detect IL-10 levels. Results In LPS group, the levels of IL-10 in serum and lung homogenates were higher than those of normal goup （ P 〈 0.01 ） ,but lower than those of LPS ＋ LA 30 mg group and LPS ＋ LA 100 mg group at 3h and 6h after LPS injection （ P 〈 0.05）. In LPS ＋ LA 100 mg group, IL- 10 levels in serum and lung homogenates at 3h and 6h were higher than those of LPS＋LA30mggoup（ P 〈0.05）.Conclusion LPScouldleadtoALI,LAcouldup-regulatelL-10 levels and improve the injury of lungs.
出处
《疑难病杂志》
CAS
2006年第5期322-324,共3页
Chinese Journal of Difficult and Complicated Cases
基金
河北省科学技术研究与发展项目(编号:052761712)
