摘要
目的探讨定量测定尿液中Survivin蛋白(存活素)在诊断膀胱移行细胞癌(BTCC)中的价值。方法以BTCC患者为研究对象,以其他泌尿系统疾病患者和正常健康成人为对照。取其尿液,离心收集上清液以酶联免疫测定法(ELISA)测定尿液中Survivin蛋白的含量,以病理学检查结果作为诊断标准,并应用受试者工作特征曲线(ROC曲线)探索尿液Survivin蛋白的参考值浓度。结果BTCC组尿液Survivin的平均含量明显高于两对照组(P<0.05),而两对照组之间尿液Survivin含量无统计学差异(P>0.05)。尿液Survivin的含量和BTCC的病理分期正相关(P<0.01);但和BTCC的病理分级无关(P>0.05)。在ROC曲线上截取值为1.35ng/ml时尿液Survivin测定诊断BTCC的敏感性和特异性分别为80%和92%。结论检测尿液Survivin含量可作为BTCC较理想的无创性诊断方法。
Objective To investigate the practicability of survivin detection in urine as a novel diagnostic method for bladder transitional cell carcinoma （ BTCC ）. Methods The quantitive enzyme - linked immunosorbent assay （ELISA） was used to detect survivin expression in urine specimens from healthy persons and patients with bladder carcinomas and other urologic diseases. The pathological examination was taken as the gold standard and ROC curve was established to evaluate the possible reference values of urine survivin. Results The average urine Survivin levels of BTCC group were significantly higher than that of two control groups （ P 〈 0. 05 both ）. Higher levels of urine Survivin were associated with BTCC of higher pathological stage （ P 〈 0.01 ）. However the average urine Survivin levels between two control groups were not significantly different （ P 〉 0. 05 ）. And the expression of Survivin didn＇t increase with BTCC grade （ P 〉 0. 05 ）. Using the cutoff value of 1.35 ng/ml, the sensitivity and specificity of the urine Survivin levels for the detection of BTCC were 80% and 92% respectively. Condusion Quantitative examination of urine Survivin with ELISA might be a potential method for the noninvasive detection of BTCC.
出处
《湘南学院学报（医学版）》
2006年第3期1-3,共3页
Journal of Xiangnan University（Medical Sciences）
基金
湘南学院科研项目(05Y048)
