宫颈癌同步放化疗前后HPV DNA及TNF-α的变化被引量：9

Changes of HPV DNA and TNF-α levels in patients with advanced cervical cancer before and after concurrent chemoradiotherapy

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摘要目的:观察中晚期宫颈癌患者同步放化疗治疗前、后宫颈分泌物HPV DNA及血清TNF-α含量的变化趋势.方法:采用第二代杂交捕获技术(HC-2)和双抗体夹心ABC-ELISA法检测30例中晚期宫颈癌患者(实验组)同步放化疗治疗前、后宫颈分泌物HPV DNA及血清TNF-α的含量,并与20例健康妇女作对照(健康对照组).结果:实验组治疗前宫颈分泌物HPV DNA阳性率为96.7%,显著高于健康对照组(10%);治疗后HPV DNA阳性率20%(P<0.01),转阴率为79.3%(23/29).实验组治疗前血清TNF-α含量为(134.15±77.99)pg/mL,治疗后为(113.14±79.99)pg/mL,均显著高于健康对照组(54.91±13.19)pg/mL,P<0.01.实验组治疗后血清TNF-α含量与治疗前比较无显著差异(P>0.05),但有下降趋势.结论:检测子宫颈癌患者宫颈分泌物HPVDNA及血清TNF-α的含量,可作为宫颈癌辅助诊断,判断治疗效果和预后的参考指标. AIM： To analyze the changing of HPV DNA in cervical secretions and serum TNF-α level in patients with advanced cervical cancer before and after concurrent ehemoradiotherapy. METHODS： The level of HPV DNA was tested by HC- 2 and TNF-α by ABC-ELISA in 30 patients with advanced cervical cancer （reseach group ） before and after chemoradiotherapy and 20 healthy women as the controls. RESULTS： The HPV infection rate before treatment（96.7% ） was higher than that after treatment （20%） and in normal controls （10%）. The negative conversion rate at the end of treatment was 79.3% （23/29）. The levels of serum TNF-α before and after chemoradiotherapy [ （ 134.15±77.99） and （ 113.14±79.99） pg/mL respectively] were both higher than that in normal controls[ （54.91±13.19 ） pg/mL, P 〈 0. 01 ]. Serum TNF-α levels had no signiificant difference between pre-treatment and post-treatment （ P 〉 0.05 ）, but a tendency of decreasing existed. CONCLUSION： The detection of HPV DNA in cervical secreations and serum TNF-α might be useful reference indexes for diagnosis and judging prognosis or curative effect.

作者曾四元李隆玉李汉萍

机构地区江西省妇幼保健院肿瘤科

出处《第四军医大学学报》北大核心 2006年第18期1717-1719,共3页 Journal of the Fourth Military Medical University

关键词中晚期宫颈癌同步放化疗 HPV TNF—α advanced cervical cancer chemoradiotherapy human papilloma virus tumor necrosis factor α

分类号 R737.33 [医药卫生—肿瘤]

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参考文献5

1Baay MF, Weyler J, Vermorken JB, et al. The value of human papillomavims detection in primary cervical cancer screening[J]. Eur J Gynaecol Oncol, 2004,25(6) :665 -669.
2赵敏,崔小丽,顾漪竹,李兆艾,严宗哲.宫颈HPV感染患者血清STNFRs、TNF水平与临床关系的研究[J].中国妇幼保健,2005,20(15):1964-1965. 被引量：4
3Chao A, Lin CT , Hsueh S, et al. Usefulness of human papillomavirus testing in the follow-up of patients with high-grade cervical intraepithelial neoplasia after conization[J]. Am J Obstet Gynecol, 2004, 190(4) :1046 - 1051.
4Deshpande A, Nolan JP, White PS, et al. TNF-alpha promoter polymorphisms and susceptibility to human papillomavirus 16-associated cervical cancer[J]. J Infect Dis, 2005,191 (6):969-976.
5韩存芝,郑曙民,荆洁线,李连青,赵先文,李新爱,孙立新,朱庆义.宫颈癌患者病毒感染及其细胞因子水平变化的调查研究[J].肿瘤防治研究,2005,32(7):442-444. 被引量：6

二级参考文献14

1宋述梅,李久香,张明和.移植性小鼠宫颈癌和增生组织中HSV－2抗原的检测[J].肿瘤防治研究,1995,22(3):144-146. 被引量：3
2李洁,刘宝印,zur Hausen H,王宏,杨学志,李力,刘文,刘洪隐,陆克昭,刘旭,牛春燕,裴润芳,苏钟浦,王言贵,董继华,张桂宁,刘春杰,许吉林,林毓纯.中国妇女宫颈癌组织中人乳头瘤病毒感染及其地理分布的调查[J].中华实验和临床病毒学杂志,1996,10(1):50-55. 被引量：69
3Aukrust P, Liabakk NB, Muller F et al. Serum levels of tumor necrosis factor and soluble TNT receptor in human immunodeficiency virus type 1 infection -correlations to clinical, immunologic and virologic parameters. J Infect Dis, 1994, 169:420.
4Diez - Ruiz A, Tilz G P, Zangerla R et al. Soluble receptors for tumor necerosis factor in clinic laboratory diagnosis. Eur J Hematol, 1995,54:1.
5Jaime B, Rosa MO, Eduardo L, et al. Asian –American variants of Human Papillomavirus 16 and risk for cervical cancer: a Case-Control Study[J]. J Natl Cancer Inst, 2001, 93(17): 1325-1329.
6Bosch TX, Manos MM, Munoz N, et al. Prevalence of Human Papillomavirus in cervical cancer: a worldwide perspective[J]. J Natl Cancer Inst, 1995, 87(10): 796-801.
7Ikenberg H, Matthay K, Schmitt B, et al. p53 Mutation and MDM2 amplification are rare even in Human Papillomavirus negative cervical carcinomas[J]. Cancer, 1995, 76(1): 57-66.
8Warren AP, Ducrog DH, Lehner PJ, et al. Human Cyomegalovirus-infectal cells have unstable assembly of major histocompatiobility compler class I Complexes and are resistant to lysis by cytoxic T Lymphocytes[J]. J of Virology, 1994, 68(5): 2822-2829.
9Sprecher E, Becker Y. Induction of interleukin-I and β gene transcription in mouse Peritoneal exudate cells of intraperitoneal infection with herpes simplex virus-I[J]. Arch Virol, 1990, 110(3): 259-269.
10Moses AV,Helen M, Garnett, et al. The effect of human Cytomegalovirus on production and biologic action of interleukin-I[J]. J Infect Dis, 1990, 162(4): 381-388.