摘要
目的研究系统性红斑狼疮(SLE)患者外周血CD4^+、CD25^+、CD4^+CD25^+淋巴细胞亚群的变化及临床意义。方法采用二色荧光抗体标记法,以流式细胞仪对34例SLE患者和18名正常人外周血CD4^+、CD25^+、CD4^+CD25^+淋巴细胞亚群进行了检测,以CD25抗原荧光强度≥10的细胞定义为CD25^(high)细胞,分析其百分率和荧光强度,并结合临床资料进行相关分析。结果活动组SLE患者外周血CD4^+CD25^+细胞、CD4^+CD25^(high)细胞占总淋巴细胞的百分率[(4．80±1．21)%和(0．25±0．10)%]均低于正常对照组[(8．92±3．21)%和(0．44±0．22)%],亦低于稳定组SLE患者[(11．28±2．09)%和(0．59±0．34)%](P均＜0．05),而稳定组SLE患者与正常对照组差异无统计学意义(P＞0．05)；但CD4^+CD25^+细胞占CD4^+细胞的比例在三组之间差异无统计学意义(P均＞0．05)；外周血CD4^+CD25^+、CD4^+CD25h^(high)细胞数与系统性红斑狼疮疾病活动指数(SLEDAI)积分呈负相关(r=-0．74,P=0．004和r=-0．614,P=0．026),与其他临床指标如补体、抗核抗体(ANA)滴度等无相关性；活动组SLE患者外周血CD4^+和CD25^+细胞亦均低于正常对照组[(23±7)vs(34±7)和(7．4±1．8)vs(13．9±3．4),P＜0．05]；SLE患者CD25抗原的荧光强度高于正常对照组(P＜0．05),而CD4抗原的荧光强度差异无统计学意义(P＞0．05)。结论活动性SLE患者外周血CD4^+ CD25^+细胞数是减少的,并与CD4^+细胞的减少有关,提示在SLE的发病及病情活动中可能起一定作用。
Objective To investigate the expression and clinical significance of peripheral blood CD4^＋ CD25^＋and CD4^＋D25^＋T subpopulations in patients with systemic lupus erythematosis. Methods The percentage and fluorescence intensities of peripheral blood CD4^＋ CD25^＋and CD4^＋D25^＋subpopulations from 34 SLE and 18 normal controls were measured with flow cytometry assay, then the correlation with clincal data was analyzed. The CD25^＋cells were defined as the CD25^high cells if their fluorescence intensity was higher than 10. Results The percentage of CD4^＋D25^＋ CD4^＋D25^high lymphocytes in active SLE patients [（4.80±1.21）% and （0.25±0.10）%] was lower than that in normal controls [（8.92±3.21）% and （0.44±0.22）% and non-active SLE patients （11.28±2.09）% and （0.59±0.34）%] （P〈0.05）. However, as for the CD25^＋cells in the CD4^＋T cells, there was no difference between SLE patients and normal control group. Peripheral blood CD4^＋CD25^＋ CD4^＋CD25^high ceils in SLE were reversely correlated with SLEDAI （r=-0.74,P=0.004 and r=-0.614, P=-0.026）, but not with others such as complements, ANA titers etc. Peripheral blood CD4^＋and CD25^＋lymphocytes in active SLE patients were also lower than those in normal controls [（23±7） vs （34±7） and （7.4±1.8） vs （13.9±3.4）, P〈0.05]. CD25 fluorescence intensities were higher in the SLE patients those in the normal controls, but CD4 fluorescence intensities were not. Conclusion CD4^＋D25^＋may play a role in the pathogenesis of SLE.
出处
《中华风湿病学杂志》
CAS
CSCD
2006年第9期555-557,共3页
Chinese Journal of Rheumatology
基金
深圳市科技和信息局基金(JH2005071141146A)
