摘要
[目的]通过对不同梅毒血清学检测程序对梅毒抗体阳性检出率影响的比较,探讨国境口岸梅毒血清学检测标准化程序。[方法]比较检测程序Ⅰ(先做甲苯胺红不加热血清试验(TRUST)。阳性血清再做梅毒螺旋体抗体明胶颗粒试验(TPPA)确认)和检测程序Ⅱ(国家质检总局推荐程序,先做TPPA检测,再做TRUST和双抗原夹心法酶联免疫吸附试验TP．ELISA检测)对出入境人员梅毒抗体阳性检出率的差异性。[结果]从2002年7月～2004年3月采用检测程序Ⅰ,共检测出入境人员10509人,检出梅毒抗体阳性63例,检出率是5．99‰。从2004年4月～2006年3月采用检测程序Ⅱ,2年共检测出入境人员10076人,TPPA阳性141人,检出率是13．99‰。经卡方检验,2种检测程序间梅毒抗体阳性检出率存在显著性差异。[结论]检测程序Ⅱ可以大大提高梅毒抗体阳性检出率,值得重视。
Objective To discuss standardizing the process of syphilis serological test . Methods The serum samples of entry-exit personnels at the ports were taken for the test by the two testing processes respectively, and the differences of the positive rate for the two test processes was compared （ process Ⅰ： TRUST test firstly, the positive serum sample will be tested by TPPA also; and processⅡ： choosing TPPA as the first method, if the test result is positive, the sample must be tested by TRUST and ELISA）. Result From July 2002 to March 2004, a total of 10 509 samples were tested by process Ⅰ. Of which, 63 were found positive , the positive rate was 5.99‰. From April 2004 to March 2006, 10 076 samples were tested by processⅡ , and 141 serum samples were positive, the positive rate was 13.99‰. There was a significant difference between the two processes according to Chi - square test（χ^2 test） . Conclusion Process Ⅱ may improve the positive inspection rate of syphilis antibody, and great importance should be attached to it.
出处
《中国国境卫生检疫杂志》
CAS
2006年第B08期65-67,共3页
Chinese Journal of Frontier Health and Quarantine
关键词
梅毒
血清学
检测
程序
标准化
Syphilis
Serological
Test
Process
Standardization
