摘要
目的研究水飞蓟宾-磷脂酰胆碱复合物(SPC)对异硫氰酸-α-萘酯(ANIT)致小鼠急性肝损伤的保护作用。方法小鼠40只随机均分成4组:正常对照组、ANIT模型组、ANIT+SPC低剂量(150 mg/kg)、ANIT+SPC高剂量(300 mg/kg)组。前两组予0.5%羧甲基纤维素钠(CMC-Na)悬液灌胃,后两组分别予SPC(150、300 mg/kg)CMC-Na悬液灌胃,连续1周。末次予SPC 12 h后,正常对照组灌胃予花生油,余3组予ANIT 60 mg/kg(花生油配制),制成小鼠急性肝损伤模型,16 h(禁食不禁水12h)后摘眼球采血,分离血清,检测血清丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)、血清总胆红素(TB)、丙二醛(MDA)、超氧化物歧化酶(SOD)水平。结果予ANIT后,模型组小鼠血清ALT、AST、TB、MDA明显升高,SOD活性明显降低;SPC能明显对抗这种改变,SPC组(1503、00 mg/kg)与模型组比较差异显著(P<0.05)。结论SPC对ANIT造成的急性肝损伤有明显的保护作用。
Objective To observe the protective effect of silybin - phosphatidylcholine compound （SPC） on acute liver damage induced by alpha - naphthylisothiocyanate（ANIT） in mice. Methods Forty mice were randomly divided into 4 groups： normal group, model group, SPC Ⅰ group, SPC Ⅱ group. Intragastric administration of 0.5 % carboxymethylcelluloes- Na（CMC - Na） suspension were given to the normal and model group,and 2 quantities of SPC suspension （ 150 and 300 mg/10g） were respectively given to SPC Ⅰ and SPC Ⅱ groups for 1 week. At 12 h after last administration of SPC, all of groups besides normal were given ANIT （ dis,solved in peanut oil, 60 mg/kg） by lavage and the normal group just only were given peanute oil by the .same volume and same way. After 16 h of ANIT administration, the levels of serum alanine transferase（ALT）, aspartate aminotransferase（AST）, serum total bilirubin（TB）, malondialdehyde （MDA） and superoxide dismutase （SOD） were detected by biochemical method. Results Serum levels of ALT, AST, TB and MDA markedly increased after ANIT was administered via intragastric tube, and the level of serum SOD also decrease. SPC could obviously inhibit the alteration of the activities of serum ALT, AST, TB, MDA and SOD（ P 〈 0.05 ）. Conclusion SPC possess a protective role on ANIT- induced hepatic damage in mice.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2006年第17期1166-1167,共2页
Journal of Applied Clinical Pediatrics
基金
河南省科技攻关项目资助(001180203)
2004年度河南省科技进步二等奖(2004-G-156-R01106)
关键词
水飞蓟宾-磷脂酰胆碱复合物
异硫氰酸-萘酯
肝损伤
保护作用
silybin - phosphatidylcholine compound
alpha - naphthylisothiocyanate
liver damage
protective effect
