摘要
以驱蚊香草的幼嫩叶片作外植体,在含不同激素的不同培养基上进行丛生芽的诱导、芽的继代增殖、芽生根与移栽研究,以快速获得再生植株。结果表明,丛生芽诱导培养基以MS+6-BA1.0mg/L+NAA0.1mg/L较好,诱导率为96%;增殖培养基以MS(B5)+6-BA0.5mg/L+NAA0.1mg/L较好,增殖系数达8倍以上,且增殖芽生长好;壮苗培养基为MS(B5)+6-BA0.1mg/L+NAA0.2mg/L为好;生根培养基以1/2MS+NAA0.1mg/L和MS+IBA0.2mg/L为最佳,生根率达100%,幼苗生长势旺。影响工厂化育苗的主要因素有激素浓度、继代次数和移栽管理。
The research made a serial of studies on buds inducement and reproduction, root differentiation and cultivation of young plant in different hormone and different culture media, which selected tender leaves of Pelargonium citrosum as explants in germfree condition. The results showed that MS＋6-BA1.0mg/ L＋NAA0.1mg/L was good for buds inducement and the rate of inducement is 96%, that MS （Bs） ＋6BA0.5mg/L ＋NAA0.1mg/L are suitable for buds reproduction, the coefficient of reproduction is up to 8, and buds grew very well, that MS （Bs）＋6-BA0.1mg/L＋NAA0.2mg/L were good for getting stronger, and that 1/2MS＋NAA0.1 mg/L and MS＋[BA0.2 mg/L were best for rootage, the rooting rate is 100% , and the young plants grew very well. The main factors affecting its large-scale production are concentration of hormone, times of subculture and transplant manage.
出处
《中国农学通报》
CSCD
2006年第9期59-62,共4页
Chinese Agricultural Science Bulletin
关键词
驱蚊香草
组织培养
工厂化快繁
影响因素
Pelargonium citrosum Vanleenii, Tissue culture, Large-scale production, Affecting factors
