摘要
Aim To determine in vitro the rat plasma protein binding rate by using microdialysis method.Methods The binding rate was determined by using microdialysis probe as sampling tools and zero-net flux method as calibrating method.The regression equation was made by the difference of concentrations between the dialysis sample and the perfusate.The（x-intercept） of regression equation was the free drug concentration（Cf）.The plasma protein binding rate was calculated by using the following equation: f=（C0-Cf）/C0.Result The binding rate was kept relatively stable in the studied concentration range.Conclusion It is feasible that the plasma protein binding rate can be determined by using microdialysis method.
Aim To determine in vitro the rat plasma protein binding rate by using microdialysis method. Methods The binding rate was determined by using microdialysis probe as sampling tools and zero-net flux method as calibrating method. The regression equation was made by the difference of concentrations between the dialysis sample and the perfusate. The x-intercept of regression equation was the free drug concentration （Cf）. The plasma protein binding rate was calculated by using the following equation： f = （Co - Cf）/C0. Result The binding rate was kept relatively stable in the studied concentration range. Conclusion It is feasible that the plasma protein binding rate can be determined by using microdialysis method.
出处
《药学学报》
CAS
CSCD
北大核心
2006年第9期909-912,共4页
Acta Pharmaceutica Sinica
基金
国家自然科学基金资助项目(30371783)
国家中医药管理局中医药科学技术研究专项资助项目(02-03ZP0047)
广东省自然科学基金资助项目(31473)
关键词
青藤碱
血浆蛋白结合率
微透析
零净通量法
sinomenine
plasma protein binding rate
microdialysis
zero-net flux method
