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依达拉奉抑制缺血再灌注肾脏ICAM-1的表达 被引量：1

Inhibitory effect of Edaravone on ICAM-1 expression in rat kidney after ischemia-reperfusion injury
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摘要 目的探讨依达拉奉对大鼠肾脏缺血再灌注后ICAM-1表达的影响。方法将24只雄性Wistar大鼠随机分为假手术组(S组)、缺血再灌注组(I组)、依达拉奉治疗组(E组),其中S组和E组大鼠均夹闭双侧肾蒂45 min、再灌注24 h制成肾缺血再灌注损伤动物模型。缺血前和再灌注即刻,E组经尾静脉给予依达拉奉3 mg/kg,S组和I组经尾静脉给予等量生理盐水。于再灌注24 h取左肾苏木精-伊红染色观察炎症细胞浸润情况,免疫组织化学观察ICAM-1表达。结果光镜下S组结构正常,I组中大量炎症细胞浸润肾间质,EB组炎症细胞浸润减少;免疫组织化学显示,I组中ICAM-1表达较S组上升,EB组则较I组下降(P<0.05)。结论依达拉奉抑制大鼠肾脏缺血再灌注损伤时ICAM-1的表达。 Objective To investigate the effects of Edaravone on intercellular adhesion molecule-1 （ICAM-1） expression in rat kidneys after ischemia-reperfusion injury. Methods Twenty-four male Wistar rats were divided randomly into 3 groups： sham-operated group （S group）, ischemia-reperfusion group （Ⅰ group ） and Edaravone treatment group （E group）. All rats, except S group with only anesthetization and leparotomy but not ischemia, were subjected to renal ischemia-reperfusion injury by clamping bilateral renal pedicles for 45 minutes followed by 24h of reperfusion. Rats were treated before and after ischemia with Edaravone to E group or saline to S and Ⅰ group through tail vein injection. After the reperfusion period of 24h, the rats were sacrificed and inflammatory cells infiltration as well as the expression of ICAM-1 in rat kidneys was observed. Results Normal morphology was observed by light microscopy in the S group. Fewer leukocytes in the E group infiltrated the kidney than that in the Ⅰ group. Expression of ICAM-1 in rat kidneys in the E group was significantly decreased than that of the Ⅰ group （P〈0. 05）. Conclusion Edaravone inhibit the expression of ICAM-1 in rat kidneys after ischemia-reperfusion iniury.
作者 徐述雄 孙兆林 何坚 刘军 冯梅 单刚 宋大龙
机构地区 贵州省人民医院泌尿外科
出处 《贵州医药》 CAS 2006年第9期774-776,共3页 Guizhou Medical Journal
基金 贵州省省长基金资助[项目编号:黔省专合字(2005)230]
关键词 依达拉奉 肾脏 缺血再灌注损伤 细胞间粘附分子-1 Edaravone Kidney Ischemia-reperfusion injury ICAM-1
分类号 R329.27 [医药卫生—人体解剖和组织胚胎学] R318.16 [医药卫生—生物医学工程]
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参考文献10

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共引文献5

同被引文献6

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贵州医药
2006年 第9期

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