摘要
用免疫组织化学方法，观察和计数了１６周ＳＤ大鼠和衰老ＳＤ大鼠（２４个月）脑血管周巨噬细胞。冷冻切片厚度为１６μｍ。结果显示，特异性巨噬细胞单抗（ＥＤ２）染色仅限于血管周巨噬细胞。ＯＸ－４２阳性的小胶质细胞与ＥＤ２阳性的细胞不同，ＦＶＩＩＩ－ＲＡ染色证实，血管内皮细胞也为ＥＤ２染色阴性。高倍显微镜下计数（每平方毫米脑组织内巨噬细胞个数）表明，衰老的ＳＤ大鼠脑血管周巨噬细胞（７．５±０．８）明显高于１６周ＳＤ大鼠（２．４±０．６，Ｐ＜０．０１）。结果提示，衰老与脑血管周巨噬细胞的增加有关。
For study of the pathology of aging and cerebrovascular diseases,the perivascular macrophages in the brains of 16-week-old SD rats and 2-year-old SD rats were investigated and counted by using the method of immunohistochemistry. The cryostat sections were cut into the thick of 16μm,The results showed that the ED2-specific staining was restricted in a perivascular location, and no ED2 staining was found in the resident microglia or in the endothelial cells,which were identified by staining with the antibodies FVIII-RA and OX-42.The number of perivascular cells per spuare milimeter of highpower field was significantly greater in 2-year-old rats(7.5±0. 8)than in 16-week-old rats(2. 4±0.6,P<0. 01 ).The results suggest that the advanced age is associated with increased accumulation of macrophages.
出处
《解剖学报》
CAS
CSCD
北大核心
1996年第1期82-84,共3页
Acta Anatomica Sinica
