摘要
用小白鼠传代取得的多房棘球蚴,人工感染家狗和家猫后,在其体内均可得到成熟的成虫。两者成虫的虫卵和孕节用以再感染小白鼠,同样都可发育为正常的多房棘球蚴。狗的粪检最早发现虫卵是在感染后的第39天,从60天后持续至90天为排卵的高峰期。感染39天的狗的剖检证明成虫主要寄生的部位是在小肠,其中回肠成虫数量最多,计1257条,其次为空肠计975条,十二指肠虫数最少,为895条。感染15天猫的剖检所见成虫寄生部位和狗基本相似,回肠虫数最高,计717条,空肠次之为611条,十二指肠计有503条。比较狗、猫所得的成虫数、孕节的含卵数、排卵的持续天数、成虫的发育状况以及成虫存活天数等分析,明显看出家狗比家猫为本虫更适宜的终宿主。 组织化学观察证明成虫和多房棘球蚴的原头节的髓质区是糖原主要的贮存所。顶突、吸盘、睾丸、卵黄腺、子宫壁、虫卵的胚胎以及育囊和不育囊的角质层、生发层等均有糖原分布,但卵巢、顶突钩和卵壳等则不含糖原。此外,顶突、吸盘、肌肉、排泄管、石灰质颗粒以及卵的胚胎等含有丰富的粘多糖和粘蛋白,PAS均呈强阳性反应,但睾丸内含量较少,卵壳呈阴性反应。
Domestic dogs and cats were infected with alveolar hydatid cysts from white mice which had been inoculated with alveolar cysts from naturally infected Myospalax f ontanieri in an endemic area in Ningxia. Results showed that mature aduts of E. multilocularis were well developed in both dog and cat. Mature alveolar hydatid cysts could be recovered when reinfection was performed on mice with gravid proglottids and eggs collected from the above mentioned dogs and cats. Eggs of E. multilocularis wese first found in Jog faces 38 days after infection, but they were most numerous within 60-90 days, and most of the adult worms were harbored in the small intestine. In an autopsy on a dog 39 days after infeetion, 1257 adults were recognized in the Heum, 975 in the jejunum and 895 in the duodenum. Besides. postmortem made on a cat infected by 15 days revcaled 717 adult worms in the ileum, 611 in the jejunum and 503 in the duodenum. Based on the comparison between these in the dog and those in the cat, of the numbers of adults and eggs the sncecssive time on egg expelling, the development and longevity of the aduk, the authers oneiaded that dogs were more suitablefor E. multilocularis to be parasitized as final hosts.
Histochemical studies indicated that the medulla of the adult and its protoscolex were main places for glycogcn storage. There was also some glycogen in the rostellum, sucker, testis, viteline gland, uterous wall, egg embryo, the laminated and germinal layers of fertile and sterile cysts, but there was a complete absence of glycogen in the ovary, rostellar hooks and egg shell. In addition, the rostellum, suckers, muscles, excretory canal, calcareous corpuscles and egg embryo were rich in mucopolysaccharide and mucin, but they were less stored in the testis and absent in the egg shell.
出处
《地方病通报》
1990年第3期46-53,135-137,共8页
Endemic Diseases Bulletin
基金
国家自然科学基金
关键词
多房棘球蚴
成虫
原头节
组织化学
Echinococcus multilocularis
Dog, cat, domestic
Adult worm
Protoscolex
Histochemistry
