摘要
目的检测抑癌基因PTEN在四种胃癌细胞中mRNA和蛋白表达水平及其5’启动子区CpG岛甲基化状态。方法采用甲基化特异性聚合酶链反应(Methylation-specific PCR,MSP)检测四种不同分化程度的胃癌细胞(HGC-27,MGC-803,BGC-823,SGC-7901)中PTEN基因启动子区域甲基化状态,RT-PCR和Westernblot法分别检测其mRNA和蛋白表达水平。结果除SGC-7901外,其他三种细胞可检测到PTEN基因启动子的甲基化。PTENmRNA和蛋白表达水平依次为:SGC-7901最高(P<0.01),BGC-823、MGC-803次之(两者间无显著性差异,P>0.05),HGC-27表达水平最低(P<0.01),其表达与细胞分化程度呈正相关趋势。结论PTEN基因启动子区异常甲基化可能导致该基因转录表达失活,使其蛋白表达减少甚至缺失,这可能是导致胃癌发生、发展的原因之一。
Objective To detect hypermethylation status of the 5＇ CpG island locating in the promoter re gion of PTEN gene and the expression level of their mRNA and protein in 4 gastric carcinoma cells. Methods Using methylation-specific PCR（MSP） technique to detect methylation status of PTEN gene in 4 different differentiation gastric cancer cells （HGC-27, MGC-803, BGC-823, SGC-7901 ）, RT-PCR and Western blot technique to detect their mRNA and protein expression level. Results Except SGC-7901, other three cells were detected hyperrnethylation of PTEN gene. The mRNA and protein expression were highest in SGC-7901 （P〈0. 01 ）, higher in BGC-823, MGC 803 （P〈0.01 ） and lowest in HGC 27 （P〈 0. 01）. The level of PTEN mRNA, protein expression and their differentiation were correlated. Conclusion Hypermethylation can inactivate the transcription of PTEN and reduce its protein expression. It may be a considerable mechanism which leads to oncogenesis, metastasis of gastric carcinoma.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2006年第9期632-634,共3页
Cancer Research on Prevention and Treatment
基金
国家自然科学基金资助项目(30300154)
关键词
胃癌
PTEN基因
甲基化
Gastric carcinoma
PTEN gene
Methylation
