摘要
目的:探讨强骨胶囊对雌激素受体(ER)活性的影响。方法:将含有ERs配体结合域(LBD)的质粒pCMV-BD和pFR-Luci报告基因及内标质粒pFRTlaczeo共转染到Hela细胞中,检测强骨胶囊与雌激素受体的关系;MTT法检测强骨胶囊对细胞活力的影响。结果:低剂量(12.5ng/ml)的强骨胶囊对两种ER均无明显效果,但是高剂量(125ng/ml和1.25μg/ml)可以显著地激活ERs,并可以显著提高细胞活性。结论:强骨胶囊治疗骨质疏松的疗效可能与激活ERs的作用有关。
Objective： To study the effects of ＂Qianggu Capsule＂ on estrogen receptors activity. Methods： pCMV-BD plasmid containing ERs ligand binding domain （LBD） and pFR-Luci report plasmid and pFRTlaczeo control plasmid were co-transfection into Hela cells, the relationship between ＂Qianggu Capsule＂ and estrogen receptors was detected by luciferase activity; the effects of ＂Qianggu Capsule＂ on cell activity were detected by MTI＂ method. Results： Low dose（12.5 ng/ml）＂Qianggu Capsule＂ had no significant effect on ERs, high-dose（125 ng/ml and 1.25 μg/ml） ＂Qianggu Capsule＂ obviously activated ERs and improved cell activity. Conclusion： The treatment of osteoporosis by ＂Qianggu Capsule＂ may be related to its effect of activating ERs.
出处
《上海中医药大学学报》
CAS
2006年第3期43-45,共3页
Academic Journal of Shanghai University of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(30400606)
广东省自然科学基金资助项目(04010036)
广东省科技厅基金资助项目(2004-139)
广州中医药大学创新基金资助项目(K004044)
关键词
强骨胶囊
质粒共转染
雌激素受体
HELA细胞
配体结合域
＂Qianggu Capsule＂
plasmid co-transfection
estrogen receptor
Hela cell
ligand binding domain
