摘要
先后用驱蚊香草的叶片、叶柄、幼嫩顶芽、休眠芽、带皮茎段、去皮茎段等作外植体,进行愈伤组织的诱导.结果表明:在所有的外植体中,以幼嫩顶芽为最好,去皮茎段、叶片次之,愈伤组织诱导率分别为94%、89%和80%;带皮茎段和叶柄的愈伤组织诱导率较低,为25.7%和14.3%左右,休眠芽未诱导出愈伤组织.
The callus were inducted on MS medium with leaves, petioles, dormant buds, apical buds, stem as explants respectively. The results indicted that the apical buds is the best among explants. The induction rates of callus from apical buds and stems excluding skin, leaves were 94%,89% respectively, with the former being obviously better than the latter, the rates of stems and petioles were 25 % and 15 %,However ,there is no callus induction from dormant buds.
关键词
驱蚊香草
愈伤组织
组织培养
外植体
Pelaronium citrosum vanleenii
tissue culture
callus
explants
