摘要
目的：建立了HSA荧光猝灭法测定Cu（Ⅱ）的分析新方法。方法：在均匀设计优化的反应条件下，研究了Cu（Ⅱ）对HSA的荧光猝灭效应。结果：在pH=8．0的KH2PO4-Na2HPO4缓冲溶液中，一定量的Cu（Ⅱ）对HSA荧光的猝灭有良好的线性关系。检出限为19．7ng／ml；相对标准偏差0．9％-1．3％；样品加标回收率93．0％～102．7％；线性范围0．07～6．00μg／ml。结论：方法与单因素轮换法实验结果比较，实验条件更优化，方法更简便、灵敏。可直接用于环境水样中Cu（Ⅱ）的测定。
Objective：To develop a new method for determination of Copper（ Ⅱ ） in environmental water with human serum albumin（HSA）. Methods：The method is based on the fluorescence quenching effect of Copper（Ⅱ） to HSA in conditions, which were determinated by uniform design experiment, such as KH2 PO4 - Na2 HPO4 buffer solution was pH = 8. 0, reaction temperature was 5℃ and volumn of 1.0 mol/L NaCI solution was 1.0 ml. Results： △I （the quenching value of Ⅰ350 nm） was linear to the concentration of Copper（ Ⅱ ） in the range of 0. 07 - 6.0 μg/ml in conditions selected（λex = 290 nm, λem = 350 nm et al. ）. The detection limit was 19. 7 ng/ml. The RSD was 0. 9% - 1.3% and the recovery was 93.0% - 102. 7%. Conclusion：These results showed much better than those of Single - factor rotates experiment. The method proposed has been successfully applied to the direct determination of Copper（ Ⅱ） in environmental water.
出处
《中国卫生检验杂志》
CAS
2006年第9期1043-1045,共3页
Chinese Journal of Health Laboratory Technology
基金
广州市海珠区立项课题(2004-D-13)
关键词
均匀设计
人血清白蛋白
荧光猝灭
铜
Uniform design
Human serum albumin
Fluorescence quenching method
Copper（ Ⅱ ）
