摘要
文章系统研究了B藻红蛋白在环境因子和变性剂作用下的特征吸收光谱和荧光发射强度的变化规律，结果表明：B藻红蛋白分别在pH4．5～9．5、温度在25-60℃和日光灯照射下（光强2800lx）24h以内相对稳定，其荧光发射强度和特征吸收光谱变化不大；分别在0．5％-3％SDS和0．25％-1％β-巯基乙醇作用下B藻红蛋白的光谱特性发生了显著变化，荧光发射强度严重衰减，特征吸收峰消失；在10％～70％乙腈或2～8mol·L^-1脲作用下，荧光发射强度有所衰减，但特征吸收光谱峰形未变化，只是峰强度略有减小。简要分析了环境因子和变性剂引起B-藻红蛋白的构象变化的可能原因。
Effects of environmental factors and denaturants on the variation rules of the specific absorption spectrum and fluores- cence emission intensity of B-phycoerythrin were systematically investigated in the present paper. The results showed that B- phycoerythrin was relatively stable under certain conditions., the pH range from 4. 5 to 9.5, temperature range from 25 to 60 ℃, and illumination intensity at 2 800 lx in 24 h. The specific absorption spectrum and fluorescence emission intensity of B-phycoerythrin changed slightly. The conformation of B-phycoerythrin changed remarkably with 0. 5%-3% SDS or 0. 25-1% β-mercaptoethanol, the fluorescence emission intensity declined greatly, and the specific absorption peaks disappeared. The fluorescence emission intensity declined, while the specific absorption spectrum did not change, but the peak intensity reduced slightly with 10%-70% acetonitrile or 2-8 mol ·L^-1 urea. The possible reasons for the conformation changes of B-phycoerythrin with various environmental factors and different denaturants were briefly discussed.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2006年第12期2258-2263,共6页
Spectroscopy and Spectral Analysis
基金
广东省自然科学基金项目(5006524)
广东省科技攻关项目(2005B20301017)资助
关键词
藻红蛋白
环境因子
变性剂
吸收光谱
荧光
Phycoerythrin
Environmental factors
Denaturant
Specific absorption spectrum
Fluorescence
