摘要
目的:观察何首乌水提液对去卵巢大鼠骨组织的动态影响。方法:把64只6月龄SD雌性大鼠随机分为4组,即假手术组、卵巢切除组、何首乌组、尼尔雌醇(E2)组,每组16只。各组大鼠分别于开始给药后第15日、第30日将各组的半数动物处死,测定骨羟脯氨酸(HOP)、骨钙、骨磷、血清及骨碱性磷酸酶(ALP)、血清及骨抗酒石酸酸性磷酸酶(TRAP)并做了骨组织的病理HE染色。结果:在两个阶段的治疗中,何首乌组与卵巢切除组比较,骨有机质和骨矿物质均有显著性升高;骨组织病理形态改善;TRAP活性变化没有出现明显改变(P>0.05),而ALP活性出现明显的变化。骨组织ALP比血清ALP的统计学差异更大,并且以上指标在第二阶段较第一阶段的统计学差异更为显著。结论:何首乌水提液可能主要通过增强ALP活性,抑制骨胶原、骨钙、骨磷的丢失,从而抑制去卵巢大鼠的骨质疏松。
Objectiv：Observing bone dynamic effect on the ovariotomized rat treated with extract ot Polygonum multifloruma.Methods：sixty-four female six months SD rats were divided into 4 groups randomly （16 rats/ group）：sham ovariectomized group （Sham） ,ovariectomied group （OVX） ,ovariectomied ＋Polygonum multifloruma group （HSW）,ovariectomized＋Nilestriol group （E2）.Thirty days after operation,HSW or E2 group were given Polygonum muhifloruma or Nilestriol respectively,Sham and OVX groups are given distilled water once a day. Half animals of each group were killed on 15th day and 30th day respectively.Serum,femurs and tibias were collected.And then following tests were made：serum and bone alkaline phosphatase （ALP）,serum and bone tartrate-resistant acid phosphatase（TRAP）,and bone hydroxyproline（HOP）,calcium（Ca）,phosphorus （P）,bone morphological features of femur.Results：Compared with OVX group,Bone organic and mineral were remarkably increased in HSW and E2 groups during the 2 terms.Bone morphological features of femur were improved.Bone TRAP activity had no obvious changes in E2 group （P〉0.05）.Bone ALP activity had obvious changes in HSW group （P〈0.05）.In addition,all the markers on 2nd term had more obvious difference than the 1st term on statistics.Conclusion：Polygonum multifloruma could promote bone ALP activity to inhibite the lost of bone organic and mineral content,and improve bone morphological features of osteoporotic femur in ovariectomied rats.
出处
《中日友好医院学报》
2006年第4期217-221,共5页
Journal of China-Japan Friendship Hospital
基金
河北省教委博士基金资助课题
关键词
卵巢切除大鼠
骨质疏松
何首乌
尼尔雌醇
骨组织
ovariectomied rat
osteoporosis
polygonum multifloruma
nilestriol
bone tissue.
