摘要
目的建立白癜风丸中阿魏酸的高效液相色谱(HPLC)含量测定方法。方法采用ODS色谱柱(4.6mm×250mm,5μm);流动相:甲醇-1%冰醋酸水溶液(1∶1);流速:1.0mL/min;柱温30℃;检测波长:320nm。结果阿魏酸在11.20～84.0μg/mL范围内呈良好线性关系(r=0.9998),平均加样回收率为98.45%,RSD为0.97%。结论HPLC法用于测定白癜风丸中阿魏酸的含量是可行的,本方法简便、快速、准确、重复性好。
Objective To establish a HPLC method for determining the ferulic acid in Baidianfeng Pill. Method ODS column （4.6 mm×250 mm, 5μm） was used, the mobile phase consisted of methanol-l% acetic acid aqueous solution（1 ： 1）. The flow rate was 1.0 mL/min and column temperature was at 30℃. The detecting wave length was at 320 nm. Results The method was linear with the range of 11.20-84.0μg/mL for ferulic acid （r=0.9 998）. The average recovery was 98.45%. Conclusion The method is simple, rapid and accurate. It can be used for quantitative detemination of ferulic acid in Baidianfeng Pill.
出处
《中国中医药信息杂志》
CAS
CSCD
2006年第9期49-50,共2页
Chinese Journal of Information on Traditional Chinese Medicine
