摘要
负链RNA病毒作为活病毒载体具有巨大的优势。本研究构建了水泡性口炎病毒印第安纳株(VSV-Ind)基因组全长cDNA克隆,建立了反向遗传操作系统并成功救获病毒。通过免疫荧光、电镜观察、RT-PCR及生长动力学研究,证实救获病毒保持典型的VSV印第安纳株特点,救获VSV的基因组内G蛋白前、后非编码区分别引入两个用于进一步构建重组病毒时外源基因插入的两个限制酶位点。本研究为新型重组活病毒疫苗载体和肿瘤治疗载体的研制及基础病毒学相关研究提供了重要的技术平台。
The negative RNA virus has many extremely attractive advantages to be the expression vector. The full-length genome cDNA clone of VSV Indiana strain was constructed, and the virus was generated by reverse genetic system. The rescued VSV was characterized by indirect immune fluorescence and electron microscope, and kept similar fast and high titer growth with parent Indiana strain. The reverse genetic system established for VSV provided a useful platform for development of novel recombinant vaccines and cancer therapy vector.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2006年第5期489-494,共6页
Chinese Journal of Preventive Veterinary Medicine
基金
国家"攻关"项目(2004BA519A19
2005BA711A10)
"973"项目(2005CB523200)资助
关键词
水泡性口炎病毒
反基因操作
vesicular stomatitis virus
reverse genetic
