摘要
目的探讨胆固醇酯转运蛋白D442→G变异对国人血脂水平的影响。方法收集天津地区189例健康人群,检测其血脂水平,并应用聚合酶链反应限制片长多态性分析方法对胆固醇酯转运蛋白D442→G突变进行检测。结果高密度脂蛋白胆固醇水平较高组与对照组D442→G等位基因频率分别为6.03%和1.53%,具有统计学差异(P<0.05)。D442→G基因变异携带组高密度脂蛋胆固醇水平显著高于对照组(P<0.01),其它血脂水平无显著性差异。结论胆固醇酯转运蛋白第15外显子D442→G突变与高密度脂蛋白胆固醇水平升高密切相关。
Aim To study the effect of the mutation D^442→G in cholesteryl ester transfer protein gene on the level of senun high density lipoprotein cholesterol. Methods 189 normal subjects were randomly collected, eholesteryl ester transfer protein geneD^442→G mutation were examined by polymerase chain reaction-restriction fragment length polymorphism （PCR- RFLP）, and the serum lipids of the subjects were determined. Results The allele frequencies of the cholesteryl ester transfer protein 15D^442→G in the group （58 subjects） with high density lipoprotein cholesterol≥1.7 mmol/L and the control （ 131 subjects） were 6.03% and 1.53% respectively （ P〈0.05）, the results showed that there was statistical difference between the two groups. The level of high density hpoprotein cholesterol in the group with the cholesteryl ester transfer protein gene exon 15 D^442→G was higher than the control group （ P〈0.01 ）. Conclusion Exon 15 D^442→G mutation in cholesteryl ester transfer protein is related to the elevated level of serum high density liporotein cholesterol.
出处
《中国动脉硬化杂志》
CAS
CSCD
2006年第1期67-69,共3页
Chinese Journal of Arteriosclerosis
