摘要
【目的】建立具有分泌乙型肝炎病毒(HBV)标志物的人肝癌裸鼠移植瘤模型。【方法】采用HBV基因转染的HePG2细胞株(2.2.15细胞)接种于4~6周龄BALB/c-nu裸小鼠项肩部皮下,观察移植瘤出现时间及测量其大小;36 d后处死裸鼠,取血检测乙肝病毒表面抗原(HBsAg)、乙肝病毒e抗原(HBeAg)和HBV-DNA等HBV标志物及肝癌相关蛋白甲胎球蛋白(AFP),并取瘤组织作病理检查。【结果】出瘤率及肿瘤出现时间与细胞接种量有关;荷瘤鼠血清HBsAg、HBeAg和HBV-DNA检测均为阳性,AFP分泌量达(350±11.5)μg/L;光镜下可见与人肝癌相似的病理改变。该模型一定程度具有类似人HBV复制的原发性肝癌形态学特征。【结论】成功建立了分泌HBV标志物的裸鼠人肝癌移植瘤模型。
[ Objective ] To establish a HBV-marker-producing nude mice model of human transplanted liver cancer （HTLC）. [ Methods] 2.2.15 cells , a HePG2 cell line transfected with HBV genome, were injected subcutaneously into athymic BALB/c-nu mice aged 4 - 6 weeks. The growth of tumor was observed every week. After 36 days the nude mice were executed and HBV markers of HBsAg, HBeAg, and HBV-DNA as well as alpha-fetoprotein （AFP） in serum were detected. The pathological features of tumor tissues were examined under light microscope. [ Results ] The incidence of HTLC and the time of HTLC appearing were correlated with the number of transplanted 2.2.15 cells. Serum HBsAg, HBeAg, and HBV-DNA were positive in the HTLC transplanted mice, the serum content of AFP was （350 ± 11.5 ） t^g/L, and pathological changes under light microscope were similar to human, indicating that the model has some similar histological characteristics of the human liver cancer infected by HBV. [ Conclusion] The nude mice model of human transplanted liver cancer, which can produce HBV markers, is established successfully in this study.
出处
《广州中医药大学学报》
CAS
2006年第5期440-441,444,共3页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
广州市科技局资助项目(编号:k4030003)
关键词
肿瘤
实验性
肿瘤病毒感染
乙型肝炎病毒
疾病模型.动物
NEOPLASMS, EXPERIMENTAL
TUMOR VIRUS INFECTIONS
HEPATITIS B VIRUS
DISEASE MODEl5, ANIMAL
