摘要
目的研究骨髓基质干细胞移植对心力衰竭不同时期内心室组织内肌动蛋白α表达量的影响。方法90只Wistar大鼠,体重180～200g,雌雄不限,清洁级动物,随机分为正常对照组(A组)、心衰组(B组)、心衰后移植BMSCs组(C组),每组30只。按3d、1周、2周、3周、4周、6周时间段,取心肌组织做免疫组织化学和Westernblott检测心肌的肌动蛋白α。结果心衰组与心衰后移植骨髓基质干细胞组的心肌型肌动蛋白α表达量在各个时间段均高于正常对照组,心衰组在各个时间段的α-actin的表达量较正常组高,分别高出对照组为:3d34%(P<0.01)、1周48%(P<0.01)、2周46.5%(P<0.01)、3周33%(P<0.01)、4周18%(P<0.05)、6周10%(P<0.05)。在心衰后BMSCs移植组分别高出对照组:3d35.6%(P<0.01)、1周53%(P<0.01)、2周57%(P<0.01)、3周52%(P<0.01)、4周45%(P<0.05)、6周40%(P<0.05)。心衰后移植骨髓基质干细胞组各时间段内的心肌型肌动蛋白α表达量均明显高于心衰组,且下降的趋势不如心衰组明显。结论骨髓基质干细胞移植可以在心肌内转化为心肌组织,延缓心衰的发展过程,骨髓基质干细胞是理想的心衰细胞治疗的种子细胞。
Objective To investigate the effect of bone marrow stromal cells（BMSCs） transplantation on myocardiM α-actin expression of heart failure different stages. Methods Ninety Wistar rats 180-200 g, clean graded and no limited gender, were randomly divided into three groups： control group（group A）, heart failure group（group B） and BMSCs transplantation group（group C）, every group was 30. Wistar-rats were sacrificed at 3d, 1 week, 2 weeks,3 weeks, 6 weeks after BMSCs transplantation, and test myocardial α-actin through immunohistochemistry and Western blot. Results The myocardium α-actin expressional level in group B and C were higher than that in group A at every stage, and both went though a tendency of heightened, The expression of α-actin in heart failure group was higher as 34%（P〈0.001） as group A at 3 day, and 48%（P〈 0.001）at 1 week, 46.5%（P〈0.001）at 2 week, 33%（P〈0.001）at 3 week, 18%（P〈0.05）at 4 week, 10%（P〈0.05）at 6 week. For group C, compared with group A, which was higher 35.6%（P〈0.001）at 3d,53%（P〈0.001）at 1 week, 57%（P〈0.001）at 2 week, 52%（P〈0.001）at 3 week, 45%（P〈0.05）at 4 week, 40%（P〈0.05）at 6 week. which descended later. But the myocardium α-actin expressional level in group C were higher than that in group B, and descending tendency was not obvious. Conclusion BMSCs which is transplanted to lesion myocardium could differentiate into myocardial cells, and defer the development of heart failure, so BMSCs is an ideal seminal cell for myocardium therapy.
出处
《解剖学研究》
CAS
2006年第3期168-171,共4页
Anatomy Research
基金
黑龙江省研究生创新科研资金项目
