摘要
目的:研究湖南汉族肾移植受者MICA008/A5.1基因与HCMV感染的关系。方法:应用PCR/SSP技术分别检测91例肾移植受者和81例湖南汉族无血缘关系的随机健康个体的MICA008/A5.1基因,同时用ELISA方法检测两组研究对象外周血中HCMV-IgM抗体。结果:健康对照组MICA008/A5.1阳性率(56.79%)比肾移植受者组(34.07%)高(P<0.05)。两组研究对象中MICA008/A5.1基因阴性个体的HCMV感染率高于该基因阳性个体的HCMV感染率(P<0.05)。结论:MICA008/A5.1基因阳性个体不容易感染HCMV,而阴性个体容易感染HCMV。
Objective To investigate the relationship between MICA ＊ 008/A5. I allele and human cytomegalovirus （ HCMV ） infection in kidney transplanted donees of Hunan Han nationality. Methods The MICA ＊ 008/A5.1 allele based on 91 kidney transplanted donees and 81 unrelated normal individuals of Han nationality in Hunan Province were analyzed by PCR/SSP assay. At the same time, anti- HCMV antibody IgM was detected in the serum by ELISA method. Results The positive rate of MICA ＊ 008/A5. 1 allele was significantly higher in the control group （ 56.79% ） than that in the kidney transplanted donee group （ 34.07% ） （ P 〈 0.05 ）. The infection rate of HCMV in those individuals whose genotype was MICA ＊ 008/A5. 1 （ - ） was significantly higher than that in the MICA ＊ 008/A5. 1 （ ＋ ）. Conclusion The individual whose genotype is MICA ＊ 008/A5.1 （ ＋ ） is not liable to HCMV infection, but the individual whose genotype is MICA ＊ 008/A5.1 （ - ） is liable to HCMV infection.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2006年第4期479-482,共4页
Journal of Central South University ：Medical Science
基金
湖南省卫生厅科研基金(C2003-003)
关键词
MICA基因
人巨细胞病毒
肾移植
MICA gene
human cytomegalovirus
kidney transplantation
