摘要
目的 :分析湖南汉族群体HLA DQA1位点等位基因多态性。方法 :应用PCR/SSP和银染PCR/SSCP技术对 60名无血缘关系湖南汉族健康个体作HLA DQA1基因分型。结果 :检出 1 0种HLA DQA1等位基因 ,基因频率范围为 0 .0 1 67～ 0 .2 2 54 ;HLA DQA1 0 30 2 , 0 50 1 , 0 1 0 2为常见等位基因 ,HLA DQA1 0 1 0 1 , 0 2 0 1 , 0 4 0 1为少见等位基因。检出 2 6种基因型 ,基因型观察值和理论值分布符合Hardy Weinberg平衡 (P >0 .0 5)。 5例样本的PCR/SSP分型只能确定一个等位基因 ,另一等位基因经PCR/SSCP确认。结论 :提供了湖南汉族群体HLA
Objective: To explore the genetic polymorphism of HLA DQA1 locus in Han nationality in Hunan province. Method: Sixty samples randomly selected from healthy individuals of Han nationality in Hunan province were typed for HLA DQA1 by PCR/SSP in combination with silver staining PCR/SSCP. Results: Ten types of HLA DQA1 alleles were found in all 60 samples, HLA DQA1*0302, *0501,*0102 were the common alleles with gene frequencies of 0.2254, 0.2254,and 0.2041 respectively .Twenty six kinds of genotypes were found ,and the genotype distribution fitted the Hardy Weinberg equilibrium test (P>0.05 ).Five samples which had ambiguity in their PCR/SSP typing were further clarified with PCR/SSCP analysis .Conclusions: Normal gene frequency values of HLA DQA1 alleles in Han nationality in Hunan province are provided; PCR/SSCP can be used to eliminate the ambiguity which might exist in PCR/SSP typing for HLA DQA1 locus. [
出处
《湖南医科大学学报》
CSCD
2000年第2期141-143,共3页
Bulletin of Hunan Medical University
基金
湖南省科学技术委员会资助课题! (0 2 961 0 5 3)
关键词
HLA-DQ抗原类
PCR
湖南
汉族
单链构像多态性
HLA-DQ antigens
polymerase chain reaction
sequence specific primer~*
single strand conformational polymorphism
