摘要
目的探讨当归注射液对辐射损伤小鼠骨髓有核细胞黏附分子表达及增殖周期的影响。方法BALB/c小鼠随机分为3组:正常组、照射组和当归注射液预防组。正常组:即未经照射组。照射组:于照射前3d起给予10ml·kg-1·d-1无菌生理盐水腹腔注射。当归注射液预防组:于照射前3d起给予腹腔注射当归注射液2·5g·kg-1·d-1。于照射后第7天断颈处死小鼠,取出股骨,计数骨髓单个核细胞。经流式细胞仪分别检测骨髓单个核细胞黏附分子CD49d和CD49e及G0/G1期细胞百分率和周期蛋白D2表达水平。结果与正常组比较,照射组造血细胞明显减少,骨髓有核细胞黏附分子CD49d和CD49e表达明显下降,G0/G1期细胞百分率明显增加,而细胞周期蛋白D2表达水平明显降低,当归注射液预防组能增加骨髓单个核细胞计数,明显提高细胞黏附分子CD49d和CD49e的表达,降低G0/G1期细胞百分率,提高细胞周期蛋白D2的表达水平。结论当归注射液能通过提高细胞黏附分子表达水平,刺激细胞周期蛋白D2的表达促进造血细胞的增殖。
Objective To investigate the influence of angelica sinenisis injection on the expression level of adherent molecule and proliferating cycle of bone marrow mononucleate cells （MNC） in irradiated mice. Methods The BALB/c mice were divided into 3 groups. Group A was normal control. Mice in group B were intraperitoneally injected with sterile saline 10 ml·kg^-1· d^-1 for 3 days, then irradiated with sub-lethal dose 6o Co γ-rays of 6 Gy. Mice in group C were intraperitoneally injected with angelica sinenisis injection 2.5 g·kg^-1 d^-1 as an intervention treatment. Mice were killed by decapitation on the 7 d after irradiation, femur were taken out for further examination. The number of femur bone marrow mononucleate cells （MNC） was counted. The CD,gd, CD49e, and cyclin De expression levels and the percentage of cells in G0/G1 in bone marrow were measured by flow cytometry. Results Compared with group A, the number of MNC, the expression levels of CD49d , CD49e , and cyclin De were obviously decreased, but the percentage of G0/G1 cells in bone marrow was significantly increased in group B （ P 〈 0.01 ） . In group C, the numbers of MNC, the levels of CD49d , CD49e, and cyclin De were significantly increased （ P 〈 0.01） and the percentage of G0/G1 cells in bone marrow were decreased （ P 〈 0.01 ）. Conclusion The study suggested that angelica sinenisis injection could promote the proliferation of bone marrow in irradiated mice by increasing the expression levels of CD49d , CD49e and cyclin D2 .
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
2006年第4期358-360,共3页
Chinese Journal of Radiological Medicine and Protection
关键词
造血细胞
细胞黏附分子
细胞周期
当归注射液
Hematopoietic cells
Intercellular adhesion molecule
Cyclin
Angelica sinenisis injection
