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mRNA展示技术 被引量：3

mRNA Display Technology
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摘要 mRNA展示技术是一种新兴的体外筛选多肽和蛋白质的有力工具.在筛选过程中,mRNA与其编码的多肽或蛋白质共价结合,形成mRNA-蛋白质融合体,能在大容量的多肽文库(1013～1015)中筛选具有特定生物学功能的多肽和蛋白质.目前,mRNA展示技术主要应用于各种靶分子的多肽和蛋白质适体的发现以及蛋白质相互作用机制的阐明和分析.由于其自身的巨大发展潜力,mRNA展示技术具有更为广阔的应用前景. mRNA display provides a new powerful tool for in vitro selecting of peptides and proteins. In the selecting process, peptides are covalently attached to their own mRNA to form mRNA-protein fusions. These mRNA-protein fusions enable in vitro selection of peptide and protein libraries of more than 1013 different sequences. The experiment conditions and protocols have been optimized in recent years. The application of mRNA display technology is mainly in the discovery of ligands for many kinds of target molecules and the analysis and mechanism elucidation on interaction between proteins. With its great potential, there will be a wide application foreground in the application ofmRNA display.
作者 张万巧 王建 贺福初
机构地区 北京放射与辐射医学研究所
出处 《生物化学与生物物理进展》 SCIE CAS CSCD 北大核心 2006年第8期795-799,共5页 Progress In Biochemistry and Biophysics
基金 国家高技术研究发展计划(2002-BA711A11 2004-BA711A19) 北京市科技计划资助项目(H030230280920).~~
关键词 体外展示技术 mRNA展示技术 mRNA-蛋白质融合体 in vitro display technology, mRNA display technology, mRNA-protein fusion
分类号 Q331 [生物学—遗传学]
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参考文献19

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同被引文献39

  • 1田志坚,张学文.体外分子展示技术[J].生命的化学,2004,24(6):518-520. 被引量：1
  • 2袁青,夏玉先,殷幼平,胡浩,王中康.文库筛选与分子进化的核糖体展示新方法[J].中国生物化学与分子生物学报,2005,21(6):732-736. 被引量：3
  • 3张永钢,李振莉.DNA展示技术的原理及应用[J].国际生物制品学杂志,2006,29(3):126-129. 被引量：1
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  • 7Shimizu Y, Inoue A, Tomari Y, et ol. Cell-free translation reconstituted with purified components. Nat Biotechnol, 2001, 19 (8):751-755.
  • 8Yan X H, Xu Z R. Ribosome-display technology:applications for directed evolution of functional proteins. Drug Discovery Today, 2006, 11(19/20): 911-916.
  • 9Spifin A S. High-throughput cell-free systems for synthesis of functionally active proteins. Trend Biotech, 2004, 22(10): 538 - 545.
  • 10Endoh T, Kanai T, Sato Y T, et ol. Cell-free protein synthesis at high temperatures using the lysate of a hyperthermophile. J Bioteehnol, 2006, 126(2): 186- 195.

引证文献3

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生物化学与生物物理进展
2006年 第8期

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