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DNA展示技术的原理及应用 被引量：1

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摘要 分子文库的展示技术是功能基因组学研究中的一个重要工具。为了避免噬菌体展示系统等体内展示技术中体内过程对展示效果的影响,人们陆续开发了多种以基因表达产物与自身DNA模板或mRNA模板特异结合为基础的无细胞展示技术,其中以DNA模板作为展示对象的DNA展示技术不仅在展示库容量方面具有相当的优势,而且操作过程简捷方便,具有广阔的开发和应用前景。
作者 张永钢 李振莉
机构地区 美国国家癌症基金会-天津大学医用抗体联合实验室
出处 《国际生物制品学杂志》 CAS 2006年第3期126-129,共4页 International Journal of Biologicals
关键词 DNA 展示 IVC 顺式
分类号 Q78 [生物学—分子生物学]
  • 相关文献

参考文献19

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二级参考文献39

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共引文献3

同被引文献11

  • 1田志坚,张学文.体外分子展示技术[J].生命的化学,2004,24(6):518-520. 被引量：1
  • 2袁青,夏玉先,殷幼平,胡浩,王中康.文库筛选与分子进化的核糖体展示新方法[J].中国生物化学与分子生物学报,2005,21(6):732-736. 被引量：3
  • 3张万巧,王建,贺福初.mRNA展示技术[J].生物化学与生物物理进展,2006,33(8):795-799. 被引量：3
  • 4Amstutz P, Forrer P, Zahnd C ,et al. In vitro display technologies: novel developments and applications.Curr Opin Biotechnol,2001(12):400
  • 5Matsuura T, Pluckthun A. Selection based on the folding properties of proteins with ribosome display. FEBS Lett 2003,539 (123):24
  • 6Schaffitzel C et al.Proc Natl Acad Sci USA, 2001,98(15):8 572-8 577
  • 7Tateyama S, Horisawa K, Takashima H, et al. Affinity selection of DNA-binding protein complexes using mRNA display. Nucleic Acids Res, 2006,34 (3):e27
  • 8Fitz Gerald K. In vitro display technologies-new tools for drug discovery. Drug Discov Today, 2000(5): 253-258
  • 9Odegrip R et al. Proc Natl Acad Sci USA,2004, 101(9): 2 806-2 810
  • 10王祥斌,孔健,黄华樑.核糖体展示技术的原理与应用[J].中华微生物学和免疫学杂志,2002,22(5):582-586. 被引量：4

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国际生物制品学杂志
2006年 第3期

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