摘要
目的 建立测定联苯双酯及滴丸含量的反相高效液相色谱法。方法 采用德国Merck公司LichrocART C18柱（250mm×4mm，5μm）为色谱柱，流动相为甲醇-水（65：35），流速为1．0mL·min^-1，检测波长278nm，柱温35℃。结果 联苯双酯在6．04～60．4pg·mL^-1浓度范围内线性关系良好（r=0．9999）。原药平均加样回收率为99．5％（RSD=0．53％，n=9），滴丸平均加样回收率为98．7％（RSD=0．85％，n=9）。结论 本法用于测定联苯双酯及其滴丸含量，简便、快速，测定结果准确。
OBJECTIVE To establish RP-HPLC method for the determination Bifendate in Bifendate material and Bifendate Pills. METHODS Using Merck KgaA LiChroCART C18 column,methanol-water（65 ： 35） as the mobile phase at a flow rate of 1.0mL · min^-1, the detection wavelength was 278nm and a column temperature at 35℃. RESULTS There was good linear relationship in the concentration range of 6.04-60. 4μg·mL^-1 （r= 0. 9999）. The material average recovery was 99.5% （RSD= 0. 53% n= 9）and pill average recovery was 98.7 （RSD = 0. 85% n = 9）. CONCLUSION This method is simple simply, fast and accurate for determining Bifendate in Bifendate material and Bifendate Pills.
出处
《海峡药学》
2006年第4期85-86,共2页
Strait Pharmaceutical Journal
