摘要
为构建角膜基础研究的稳定载体和操作平台,通过合法渠道获得人胚胎,并对角膜上皮细胞进行了分离培养。经反复传代,初步建立了人胚胎角膜上皮细胞系,并对其生物学特性进行了研究。结果显示,培养细胞具有较典型的上皮细胞特征。细胞生长较快,最快时两天可传代一次。K19和PCNA在各代胚胎角膜细胞均有表达,K19的表达部位位于胞浆,而PCNA的表达部位位于胞核。处于细胞周期中S期的细胞比例大约为11%￣23%。染色体核型分析表明各代细胞染色体的数目和形态相似。因此,人胚胎角膜上皮细胞适合于建立相对稳定的细胞系。培养细胞具有分化上的幼稚性和较强且稳定的增殖能力,细胞生长状态良好,而且该细胞系的遗传特征较为稳定。
n order to construct the stable supporter and work platform for corneal basic research, corneal epithelial cells were isolated and cultured from a human embryo acquired by legal way. After human embryo corneal epithelial cell line was established through many cell generations, its biological character was researched. The results showed that cultured cells had typical character of epithelial cells. The cells grew fast and its maximal subculture frequency to go down to the future generation was 2 days. The expressions of K19 and PCNA could be found in embryo corneal epithelial cells in every generation, The K19 expression located in cytoplasm while PCNA expression located in nucleus. The proportion of cells in S phase of cell cycle was approximately 11%-23%. The chromosome karyotype analysis manifested that the number and morphous of chromosomes were similar in every generation cells, Thus, the human embryo corneal epithelial cells was fit for establishing a relative stable cell line, The cultured cells had early stage character in differentiation and reproductive activity with strong and stable character, The good condition existed in the course of cell growth, Furthermore, the genetic character of this cell line showed relative stable.
出处
《细胞生物学杂志》
CSCD
2006年第4期587-590,共4页
Chinese Journal of Cell Biology
基金
重庆市自然科学基金资助项目(No.2004BB5251)~~
关键词
人胚胎
角膜上皮细胞系
生物学特性
human embryo
corneal epithelial cell line
biological character
