摘要
用稀碱处理大马哈鱼皮,然后进行限制性酶解,制备得到胶原蛋白,检验制备样品的纯度,测定样品的热变性温度和细胞相容性。制备的胶原蛋白α链的含量≥75%,β链的含量≤25%,有微量的γ链;变性温度是12.5℃;细胞培养3天的相对活力是80.0±3.6%;大马哈鱼皮胶原蛋白为基质的细胞活力仅次于对照组(空白细胞培养板),优于聚乳酸、聚羟基丁酸和聚-3-羟基丁酸戊酸。海鱼皮胶原蛋白可进一步应用于生物材料研究。
By soaking the big pacificsalmon （Oncorhynchus keta ） skin in the alkali solution and then limit-digesting it in pepsin solution, the collagen was obtained. After characterizing its purity, thermal denatured temperature, and cell compatibility were determined. The results showed that fabricated collagen was composed of a chain （above 75%）, β chain （below 25%）; and with a little γ chain. Its thermal denature temperature was 12. 5 ℃. The relative activity of the cell cultured for three days on the collagen matrix was 80. 0±3. 6%. The cell activity cultured on the collagen was higher than that on polylactic acid, polyoxyhydrobutyric acid, and poly-3-oxyhydrobutyrate-co-valeric acid. The fabricated collagen as a biomaterial can be further studied.
出处
《药物生物技术》
CAS
CSCD
2006年第4期290-292,共3页
Pharmaceutical Biotechnology
关键词
海鱼皮胶原蛋白
变性温度
细胞相容性
Marine fish collagen, Thermal denature temperature, Cell compatibility
