摘要
应用HB_sA_g采取两种不同的免疫方法免疫小鼠后。取免疫的脾细胞与小鼠骨髓瘤细胞Sp2/0融合,筛选出两株抗HB_s的单克隆抗体杂交瘤细胞株。常规免疫方法获得的细胞株为WHB_s1,分泌IgG1。脾内免疫方法获得的细胞株WHB_s2,分泌IgM和IgG_(2a)抗体。经过3个月的连续培养,能稳定分泌效价高,特异性强的单克隆抗体。
Using two different immunization methods, two hybridoma cell lines(WHBs1, WHBs2) secreting antibodies against HBsAg were established by fusing mur-ine myeloma cell SP2/0 with spleen cells from BALB/c mice immunized with HBsAg. WHBsl, which was established with conventional immunizing method, is able to secret IgGl antibody, and WHBs2, which was established with intra spleen immunizing method, has the capacity to secrete both IgG2a and IgM antibodies. The specificity and titer of the monoclonal antibodies were not influenced for 3 months in culture.
关键词
双分泌
乙型肝炎
表面抗原
单克隆抗体
HBsAg
anti-HBs McAb
double secretjimmunization, intra spleen
