摘要
目的:观察黄芪注射液对糖化终产物(AGEs)培养大鼠肾小球系膜细胞(GMC)形态学、细胞周期及氧化应激的影响。方法:用含AGEs的培养液配制黄芪注射液、氨基胍,同时设对照。GMC培养48 h后,加混合荧光染液进行染色,并立即进行荧光显微观察。另用流式细胞仪分析GMC的周期。试剂盒测定细胞上清液SOD、MDA和GSH-PX,流式细胞仪检测细胞内ROS含量。结果:形态学显示,正常GMC形态结构正常。而GMC在AGEs的刺激下,细胞数量明显增多,细胞结构不甚清晰,细胞S期延长,G0/G1缩短,细胞内SOD、GSH-PX下调,ROS、MDA上调。在系统中加入黄芪注射液后细胞异常增殖明显减少,细胞形态趋于正常,使S期细胞减少,SOD、GSH-PX活力提高,ROS、MDA含量降低。结论:黄芪注射液能对抗AGEs对GMC的损伤作用。其可能是通过抑制氧化应激来保护GMC免受AGEs的损害。
AIM： To investigate the influence of Astragalus Injection on morphology, cell cycle and oxidative stress of rat＇s glomerular mesangial cells（ GMC ） cultured with advanced glycation end products（AGEs）. METHODS： GMC were incubated in culture medium containing AGEs in the presence of Astragalus Injection and aminoguanidin for 48 h. At the same time, the normal and control groups were established. Then the cultured GMC was stained by mixed fluorescence liquid and observed under fluorescence microscope. Cell cycle of GMC was analyzed using flowcytometry. The activity of SOD,MDA and GSH-PX in GMC supernatant were measured by test kit. The level of ROS was detected by flowcytometry. RESULTS： Morphology analysis showed that the morphology and structure of normal GMC were normal. The structure of most cells in AGEs was unclear, cell counts increased markedly and they grew intensively. Cell cycle analysis showed that cell percentage of S phase increased and G0/G1 reduced. The level of ROS, MDA remarkably increased, and SOD, GSH-PX activity reduced. Whereas Injection was added, cell morphology tended to be basically normal and cell counts decreased, the perc Astragalus entage of S phase also decreased. The level of ROS, MDA and the SOD, GSH-PX activity restored in comparison with the control groups. CONCLUSION： Astragalus Injection can prevent GMC from lesion caused by AGEs. Astragalus Injection may protect GMC from retarding the progression of diabetic nepropathy by partially inhibiting the occurrence of oxidative stress.
出处
《中成药》
CAS
CSCD
北大核心
2006年第8期1170-1174,共5页
Chinese Traditional Patent Medicine
基金
江苏省科技厅自然科学基金资助项目(BK2001127)
