摘要
采用FLUTAX直接荧光标记和抗α微管蛋白抗体的间接免疫荧光标记显示,原生动物贻贝棘尾虫(Stylonychia mytilus)细胞微管胞器由口围带、波动膜、额腹横棘毛、左右缘棘毛、背纤毛等纤毛器微管骨架、纤毛器基部附属微管和其他皮层微管骨架组成。纤毛器微管骨架和基部附属微管按皮层纤毛模式定位;皮层左、右侧微管带和领肋壁微管等其他皮层微管构成细胞特定位置的皮层微管骨架,并可能为具有背腹分化的腹毛目纤毛虫所特有,对维持细胞背腹面的形态、支持附近纤毛器(如左、右缘棘毛)的运动起作用。本文较完整地阐述了其细胞骨架的三维构形,对于深入了解纤毛虫细胞微管骨架的结构和分布特征,进一步揭示微管类胞器的功能是有意义的。
Microtubular organelles of protozoa, Stylonychia mytilus, was analyzed with anti-a-tubulin antibody and FLUTAX. The results showed that the microtubular organelles consisted of ciliatures and its base-associated microtubules as well as cortex microtubular cytoskeletons. The ciliatures include adoral zone of membranelles （AZM）, undulating membranes （UM）, frontal-ventral-transverse cirri （FVTC）, left- and fight-marginal cirri （LRMC）, dorsal kineties （DK）, etc. The ciliatures and base-associated microtubules arrayed in the specific areas of cortex. Meanwhile, the left- and right-microtubular ribbons, oral rib-wall microtubules and other microtubules composed the cortical microtubular cytoskeletons in fixed areas and they might be owned especially by the hypotrich ciliates which had dorsoventral differentiation. Moreover, they might function in the cortex-shaping and support the movement of ciliary organelles nearby. This paper describes a three-dimension network composed of microtubular cytoskeletons and provides valuable references for a better understanding of the structure characteristics and functions of microtubular cytoskeletons in ciliates.
出处
《动物学杂志》
CAS
CSCD
北大核心
2006年第4期71-76,共6页
Chinese Journal of Zoology
基金
国家自然科学基金资助项目(No.30470201)
福建师范大学校扶苗基金资助项目(No.FM001)
关键词
贻贝棘尾虫
微管胞器
荧光标记
Stylonychia mytilus
Microtubular organelles
Fluorescent labeling
