摘要
大麦（Ｈｏｒｄｅｕｍ ｖｕｌｇａｒｅ）幼穗经添加０．５ ｍ ｏｌ／Ｌ蔗糖的２ ｄ 预培养，用玻璃化保护剂ＰＶＳ２ 冰浴处理５ ｍ ｉｎ，直接投入液氮贮存；快速化冻，洗去保护剂后恢复培养，三个品种“８１Ｇ１”、“戈贝纳”和“旭９”的存活率分别为１００％ 、８２．５％ 和５０％ 。存活的幼穗能形成愈伤组织并再生植株。不经预培养，几个品种的幼穗均不能存活。存活率低的品种，其抗脱水力和抗冻力均较差。和传统的二步冷冻法相比，玻璃化法具有存活率高。
Vitrification is a new technique of cryopreservation.Immature inflorescences of barley (Hordeum Vulgare L.) were precultured in medium supplemented with 0.5 mol/L sucrose for 2 days and dehydrated in PVS2 at 0 ℃ for 5 min before plunged into liquid nitrogen.After rapid warming and unloading,the survival of three cultivars“81G1”,“Gebeina”,and “Xu 9” was 100%,82.5% and 50% respectively.Callus formation and plant regeneration was achieved from recovered inflorescences.The non precultured samples could not survival in liquld nitrogen.Desiccation tolerance and freeze tolerance affected plant survival.Vitrification shared the advantages of high levels of survival,short period of lag stage and complete viability of cryopreserved organs.
基金
国家自然科学基金
关键词
大麦
幼穗
玻璃化
超低温保存
植株再生
Hordeum vulgare
Immature inflorescences
Vitrification
Cryopreservation
