摘要
目的探讨血清中转化生长因子β1(TGF-β1)的浓度与其-509位点基因多态性的关系在煤工尘肺发生发展中的作用。方法选择110例汉族煤工尘肺患者和110例煤尘接触者为研究对象,采集外周静脉血,应用TGF-β1酶联免疫吸附试验(ELISA)试剂盒检测血清TGF-β1浓度,应用多聚酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测TGF-β1基因-509位点基因型。结果病例组煤工尘肺患者血清TGF-β1平均浓度为(65.108±29.354)pg/L,对照组为(57.296±16.603)pg/L,两者比较差异有统计学意义(P<0.05)。病例组与对照组内各基因型组血清TGF-β1平均浓度差异有统计学意义(P<0.05)。进一步用q检验两两比较,各组差异均有统计学意义(P<0.05)。TGF-β1-509位点为C/C、C/T基因型的两组血清TGF-β1的平均浓度之间差异无统计学意义(P>0.05);TGF-β1-509位点为T/T基因型的两组血清TGF-β1的平均浓度之间差异有统计学意义(P<0.05)。结论煤工尘肺患者血清TGF-β1浓度与TGF-β1基因-509位点多态性可能有关。
Objective To explore the role of the concentration of transforming growth factor I （TGF-β1） in serum and its gene polymorphisms in the development of coal worker＇ s pneumoconiosis （CWP）. Methods 110 eases of CWP and 110 coal mine workers （controls） were selected. Peripheral vein blood was drawn from every subject. With TGF-β1 ELISA reagent kit, the concentration of TGF-β1 in serum was tested. Polymerse chain reaction-restriction fragment length polymorphisms （ PCR- RFLP） techniques were used to analyze TGF-β1gene -509 site polymorphisms. Results The mean value of TGF-β1 concentrations in serum of CWP group was （65. 108 ±29. 354） pg/L, and the eontrlos was （57. 296 ±16. 603 ） pg/L, showing significant difference （ P 〈 0. 05 ）. Between CWP and the controls , significant differences was shown in the concentrations of the three gene types（P 〈0. 05） and in the concentrations ofT/T gene type （P 〈0.05）, but not in C/C and C/T（P〉0.05）. Conclusion The concentration of TC, F-β1 in serum might be related to its gene -509 site polymorphisms.
出处
《中国职业医学》
CAS
北大核心
2006年第4期265-268,共4页
China Occupational Medicine
